6GPU

Crystal structure of miniSOG at 1.17A resolution

6GPU の概要

• エントリーDOI: 10.2210/pdb6gpu/pdb
• 分子名称: Phototropin-2, FLAVIN MONONUCLEOTIDE, CHLORIDE ION, ... (7 entities in total)
• 機能のキーワード: singlet oxygen generator, fluorescent protein, fmn, flavoprotein
• 由来する生物種: Arabidopsis thaliana (Thale cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14021.30

構造登録者

Lafaye, C.,Signor, L.,Aumonier, S.,Shu, X.,Gotthard, G.,Royant, A. (登録日: 2018-06-07, 公開日: 2019-02-27, 最終更新日: 2024-01-17)

主引用文献

Torra, J.,Lafaye, C.,Signor, L.,Aumonier, S.,Flors, C.,Shu, X.,Nonell, S.,Gotthard, G.,Royant, A.
Tailing miniSOG: structural bases of the complex photophysics of a flavin-binding singlet oxygen photosensitizing protein.
Sci Rep, 9:2428-2428, 2019

PubMed Abstract: miniSOG is the first flavin-binding protein that has been developed with the specific aim of serving as a genetically-encodable light-induced source of singlet oxygen (O). We have determined its 1.17 Å resolution structure, which has allowed us to investigate its mechanism of photosensitization using an integrated approach combining spectroscopic and structural methods. Our results provide a structural framework to explain the ability of miniSOG to produce O as a competition between oxygen- and protein quenching of its triplet state. In addition, a third excited-state decay pathway has been identified that is pivotal for the performance of miniSOG as O photosensitizer, namely the photo-induced transformation of flavin mononucleotide (FMN) into lumichrome, which increases the accessibility of oxygen to the flavin FMN chromophore and makes protein quenching less favourable. The combination of the two effects explains the increase in the O quantum yield by one order of magnitude upon exposure to blue light. Besides, we have identified several surface electron-rich residues that are progressively photo-oxidized, further contributing to facilitate the production of O. Our results help reconcile the apparent poor level of O generation by miniSOG and its excellent performance in correlative light and electron microscopy experiments.

PubMed: 30787421
DOI: 10.1038/s41598-019-38955-3

実験手法

X-RAY DIFFRACTION (1.17 Å)