6GO0

PLANTARICIN S-B IN 100 MM DPC MICELLES. THIS IS THE BETA PART OF THE BACTERIOCIN PLANTARICIN S

6GO0 の概要

• エントリーDOI: 10.2210/pdb6go0/pdb
• NMR情報: BMRB: 34279
• 分子名称: Plantaricin S beta protein (1 entity in total)
• 機能のキーワード: antimicrobial protein bacteriocin menbrane interacting peptide, antimicrobial protein
• 由来する生物種: Lactobacillus plantarum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2877.26

構造登録者

Ekblad, B.,Kristiansen, P.E. (登録日: 2018-06-01, 公開日: 2019-03-06, 最終更新日: 2024-06-19)

主引用文献

Ekblad, B.,Kristiansen, P.E.
NMR structures and mutational analysis of the two peptides constituting the bacteriocin plantaricin S.
Sci Rep, 9:2333-2333, 2019

PubMed Abstract: The structure of the individual peptides of the two-peptide bacteriocin plantaricin S, an antimicrobial peptide produced by a Lactobacillus plantarum strain, has been determined in DPC micelles. The two peptides of plantaricin S, Pls-α and Pls-β, form an α-helix from and including residue 8 to 24 with a less structured region around residue 16-19 and an amphiphilic α-helix from and including residue 7 to 23, respectively. Activity assays on single amino acid-substituted GxxxG and GxxxG-like motifs show that substituting the Ser and Gly residues in the GxxxG motif in Pls-α and the SxxxG motif in Pls-β reduced or drastically reduced the antimicrobial activity. The two-peptide bacteriocin muricidin contains GxxxG-like motifs at similar positions and displays 40-50% amino acid identity with plantaricin S. Activity assays of combinations of the peptides that constitute the bacteriocins plantaricin S and muricidin show that some combinations are highly active. Furthermore, sequence alignments show that the motifs important for plantaricin S activity align with identical motifs in muricidin. Based on sequence comparison and activity assays, a membrane-inserted model of plantaricin S in which the two peptides are oriented antiparallel relative to each other and where the GxxxG and GxxxG-like motifs important for activity come close in space, is proposed.

PubMed: 30787405
DOI: 10.1038/s41598-019-38518-6

実験手法

SOLUTION NMR