6GK5

Crystal structure of cytochrome P450 CYP267B1 from Sorangium cellulosum So ce56

6GK5 の概要

• エントリーDOI: 10.2210/pdb6gk5/pdb
• 分子名称: Cytochrome P450 CYP267B1 protein, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: bacterial proteins, sorangium cellulosum, cytochrome p-450 enzyme system, cytochrome p450, hydroxylation, heme, oxidation-reduction, flavanone, myristic acid, tetradecanoic acid, biocatalysis, oxidoreductase
• 由来する生物種: Sorangium cellulosum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 47262.16

構造登録者

Jozwik, I.K.,Thunnissen, A.M.W.H. (登録日: 2018-05-18, 公開日: 2018-08-08, 最終更新日: 2024-01-17)

主引用文献

Jozwik, I.K.,Litzenburger, M.,Khatri, Y.,Schifrin, A.,Girhard, M.,Urlacher, V.,Thunnissen, A.W.H.,Bernhardt, R.
Structural insights into oxidation of medium-chain fatty acids and flavanone by myxobacterial cytochrome P450 CYP267B1.
Biochem. J., 475:2801-2817, 2018

PubMed Abstract: Oxidative biocatalytic reactions performed by cytochrome P450 enzymes (P450s) are of high interest for the chemical and pharmaceutical industries. CYP267B1 is a P450 enzyme from myxobacterium So ce56 displaying a broad substrate scope. In this work, a search for new substrates was performed, combined with product characterization and a structural analysis of substrate-bound complexes using X-ray crystallography and computational docking. The results demonstrate the ability of CYP267B1 to perform in-chain hydroxylations of medium-chain saturated fatty acids (decanoic acid, dodecanoic acid and tetradecanoic acid) and a regioselective hydroxylation of flavanone. The fatty acids are mono-hydroxylated at different in-chain positions, with decanoic acid displaying the highest regioselectivity towards ω-3 hydroxylation. Flavanone is preferably oxidized to 3-hydroxyflavanone. High-resolution crystal structures of CYP267B1 revealed a very spacious active site pocket, similarly to other P450s capable of converting macrocyclic compounds. The pocket becomes more constricted near to the heme and is closed off from solvent by residues of the F and G helices and the B-C loop. The crystal structure of the tetradecanoic acid-bound complex displays the fatty acid bound near to the heme, but in a nonproductive conformation. Molecular docking allowed modeling of the productive binding modes for the four investigated fatty acids and flavanone, as well as of two substrates identified in a previous study (diclofenac and ibuprofen), explaining the observed product profiles. The obtained structures of CYP267B1 thus serve as a valuable prediction tool for substrate hydroxylations by this highly versatile enzyme and will encourage future selectivity changes by rational protein engineering.

PubMed: 30045877
DOI: 10.1042/BCJ20180402

実験手法

X-RAY DIFFRACTION (1.6 Å)