6GJ3

The baseplate complex from the type VI secretion system

6GJ3 の概要

• エントリーDOI: 10.2210/pdb6gj3/pdb
• EMDBエントリー: 0010
• 分子名称: TssG, TssK (2 entities in total)
• 機能のキーワード: secretion, baseplate, complex, structural protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 329978.83

構造登録者

Rapisarda, C.,Fronzes, R. (登録日: 2018-05-15, 公開日: 2018-10-17, 最終更新日: 2024-05-15)

主引用文献

Cherrak, Y.,Rapisarda, C.,Pellarin, R.,Bouvier, G.,Bardiaux, B.,Allain, F.,Malosse, C.,Rey, M.,Chamot-Rooke, J.,Cascales, E.,Fronzes, R.,Durand, E.
Biogenesis and structure of a type VI secretion baseplate.
Nat Microbiol, 3:1404-1416, 2018

PubMed Abstract: To support their growth in a competitive environment and cause pathogenesis, bacteria have evolved a broad repertoire of macromolecular machineries to deliver specific effectors and toxins. Among these multiprotein complexes, the type VI secretion system (T6SS) is a contractile nanomachine that targets both prokaryotic and eukaryotic cells. The T6SS comprises two functional subcomplexes: a bacteriophage-related tail structure anchored to the cell envelope by a membrane complex. As in other contractile injection systems, the tail is composed of an inner tube wrapped by a sheath and built on the baseplate. In the T6SS, the baseplate is not only the tail assembly platform, but also docks the tail to the membrane complex and hence serves as an evolutionary adaptor. Here we define the biogenesis pathway and report the cryo-electron microscopy (cryo-EM) structure of the wedge protein complex of the T6SS from enteroaggregative Escherichia coli (EAEC). Using an integrative approach, we unveil the molecular architecture of the whole T6SS baseplate and its interaction with the tail sheath, offering detailed insights into its biogenesis and function. We discuss architectural and mechanistic similarities but also reveal key differences with the T4 phage and Mu phage baseplates.

PubMed: 30323254
DOI: 10.1038/s41564-018-0260-1

実験手法

ELECTRON MICROSCOPY (4.3 Å)