6GII

The crystal structure of Tepidiphilus thermophilus P450 heme domain

6GII の概要

• エントリーDOI: 10.2210/pdb6gii/pdb
• 分子名称: Cytochrome P450, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: cytochrome p450 class vii, oxidoreductase
• 由来する生物種: Tepidiphilus thermophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55562.46

構造登録者

Levy, C.W. (登録日: 2018-05-11, 公開日: 2018-05-30, 最終更新日: 2024-01-17)

主引用文献

Tavanti, M.,Porter, J.L.,Levy, C.W.,Gomez Castellanos, J.R.,Flitsch, S.L.,Turner, N.J.
The crystal structure of P450-TT heme-domain provides the first structural insights into the versatile class VII P450s.
Biochem. Biophys. Res. Commun., 501:846-850, 2018

PubMed Abstract: The first crystal structure of a class VII P450, CYP116B46 from Tepidiphilus thermophilus, has been solved at 1.9 Å resolution. The structure reveals overall conservation of the P450-fold and a water conduit around the I-helix. Active site residues have been identified and sequence comparisons have been made with other class VII enzymes. A structure similarity search demonstrated that the P450-TT structure is similar to enzymes capable of oxy-functionalization of fatty acids, terpenes, macrolides, steroids and statins. The insight gained from solving this structure will provide a guideline for future engineering and modelling studies on this catalytically promiscuous class of enzymes.

PubMed: 29738765
DOI: 10.1016/j.bbrc.2018.05.014

実験手法

X-RAY DIFFRACTION (1.9 Å)