6GHQ

HtxB D206N protein variant from Pseudomonas stutzeri in a partially open conformation to 1.53 A resolution

6GHQ の概要

• エントリーDOI: 10.2210/pdb6ghq/pdb
• 関連するPDBエントリー: 6EMN
• 分子名称: Probable phosphite transport system-binding protein HtxB, FORMIC ACID, 1,2-ETHANEDIOL, ... (6 entities in total)
• 機能のキーワード: periplasmic binding protein, phosphite, hypophosphite, abc-transporter, transport protein
• 由来する生物種: Pseudomonas stutzeri (Pseudomonas perfectomarina)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30809.01

構造登録者

Bisson, C.,Robertson, A.J.,Hitchcock, A.,Adams, N.B. (登録日: 2018-05-08, 公開日: 2019-05-22, 最終更新日: 2024-01-17)

主引用文献

Adams, N.B.P.,Robertson, A.J.,Hunter, C.N.,Hitchcock, A.,Bisson, C.
Phosphite binding by the HtxB periplasmic binding protein depends on the protonation state of the ligand.
Sci Rep, 9:10231-10231, 2019

PubMed Abstract: Phosphorus acquisition is critical for life. In low phosphate conditions, some species of bacteria have evolved mechanisms to import reduced phosphorus compounds, such as phosphite and hypophosphite, as alternative phosphorus sources. Uptake is facilitated by high-affinity periplasmic binding proteins (PBPs) that bind cargo in the periplasm and shuttle it to an ATP-binding cassette (ABC)-transporter in the bacterial inner membrane. PtxB and HtxB are the PBPs responsible for binding phosphite and hypophosphite, respectively. They recognize the P-H bond of phosphite/hypophosphite via a conserved P-H...π interaction, which confers nanomolar dissociation constants for their respective ligands. PtxB also has a low-level binding affinity for phosphate and hypophosphite, whilst HtxB can facilitate phosphite uptake in vivo. However, HtxB does not bind phosphate, thus the HtxBCDE transporter has recently been successfully exploited for biocontainment of genetically modified organisms by phosphite-dependent growth. Here we use a combination of X-ray crystallography, NMR and Microscale Thermophoresis to show that phosphite binding to HtxB depends on the protonation state of the ligand, suggesting that pH may effect the efficiency of phosphite uptake by HtxB in biotechnology applications.

PubMed: 31308436
DOI: 10.1038/s41598-019-46557-2

実験手法

X-RAY DIFFRACTION (1.53 Å)