6GGZ

NMR structure of the scorpion toxin AmmTx3

Summary for 6GGZ

• Entry DOI: 10.2210/pdb6ggz/pdb
• NMR Information: BMRB: 34268
• Descriptor: Potassium channel toxin alpha-KTx 15.3 (1 entity in total)
• Functional Keywords: scorpion toxin csab fold, toxin
• Biological source: Androctonus mauritanicus mauritanicus (Scorpion)
• Total number of polymer chains: 1
• Total formula weight: 3834.56

Authors

Landon, C.,Meudal, H. (deposition date: 2018-05-04, release date: 2019-01-30, Last modification date: 2024-10-16)

Primary citation

Zoukimian, C.,Meudal, H.,De Waard, S.,Ouares, K.A.,Nicolas, S.,Canepari, M.,Beroud, R.,Landon, C.,De Waard, M.,Boturyn, D.
Synthesis by native chemical ligation and characterization of the scorpion toxin AmmTx3.
Bioorg. Med. Chem., 27:247-253, 2019

PubMed Abstract: The scorpion toxin AmmTx3 is a specific blocker of K4 channels. It was shown to have interesting potential for neurological disorders. In this study, we report the first chemical synthesis of AmmTx3 by using the native chemical ligation strategy and validate its biological activity. We determined its 3D structure by nuclear magnetic resonance spectroscopy, and pointed out that AmmTx3 possesses the well-known CSαβ structural motif, which is found in a large number of scorpion toxins. Overall, this study establishes an easy synthetic access to biologically active AmmTx3 toxin.

PubMed: 30529150
DOI: 10.1016/j.bmc.2018.12.009

Experimental method

SOLUTION NMR