6GG9

Crystal structures of a short blue light photoreceptor protein PpSB1-LOV mutant (dark state) - R61H/R66I

6GG9 の概要

• エントリーDOI: 10.2210/pdb6gg9/pdb
• 分子名称: Sensory box protein, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: blue light photoreceptor, sensory box protein, signaling protein
• 由来する生物種: Pseudomonas putida
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 76044.36

構造登録者

Roellen, K.,Granzin, J.,Batra-Safferling, R. (登録日: 2018-05-03, 公開日: 2018-07-25, 最終更新日: 2024-01-17)

主引用文献

Rollen, K.,Granzin, J.,Batra-Safferling, R.,Stadler, A.M.
Small-angle X-ray scattering study of the kinetics of light-dark transition in a LOV protein.
PLoS ONE, 13:e0200746-e0200746, 2018

PubMed Abstract: Light, oxygen, voltage (LOV) photoreceptors consist of conserved photo-responsive domains in bacteria, archaea, plants and fungi, and detect blue-light via a flavin cofactor. We investigated the blue-light induced conformational transition of the dimeric photoreceptor PpSB1-LOV-R66I from Pseudomonas putida in solution by using small-angle X-ray scattering (SAXS). SAXS experiments of the fully populated light- and dark-states under steady-state conditions revealed significant structural differences between the two states that are in agreement with the known structures determined by crystallography. We followed the transition from the light- to the dark-state by using SAXS measurements in real-time. A two-state model based on the light- and dark-state conformations could describe the measured time-course SAXS data with a relaxation time τREC of ~ 34 to 35 min being larger than the recovery time found with UV/vis spectroscopy. Unlike the flavin chromophore-based UV/vis method that is sensitive to the local chromophore environment in flavoproteins, SAXS-based assay depends on protein conformational changes and provides with an alternative to measure the recovery kinetics.

PubMed: 30011332
DOI: 10.1371/journal.pone.0200746

実験手法

X-RAY DIFFRACTION (2.04 Å)