6GG5

Crystal structure of M2 PYK in complex with Tryptophan.

6GG5 の概要

• エントリーDOI: 10.2210/pdb6gg5/pdb
• 分子名称: Pyruvate kinase PKM, PHOSPHATE ION, TRYPTOPHAN, ... (5 entities in total)
• 機能のキーワード: glycolysis, pyruvate kinase activity, transferase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 242550.06

構造登録者

McNae, I.W.,Yuan, M.,Walkinshaw, M.D. (登録日: 2018-05-02, 公開日: 2018-05-23, 最終更新日: 2024-11-20)

主引用文献

Yuan, M.,McNae, I.W.,Chen, Y.,Blackburn, E.A.,Wear, M.A.,Michels, P.A.M.,Fothergill-Gilmore, L.A.,Hupp, T.,Walkinshaw, M.D.
An allostatic mechanism for M2 pyruvate kinase as an amino-acid sensor.
Biochem. J., 475:1821-1837, 2018

PubMed Abstract: We have tested the effect of all 20 proteinogenic amino acids on the activity of the M2 isoenzyme of pyruvate kinase (M2PYK) and show that, within physiologically relevant concentrations, phenylalanine, alanine, tryptophan, methionine, valine, and proline act as inhibitors, while histidine and serine act as activators. Size exclusion chromatography has been used to show that all amino acids, whether activators or inhibitors, stabilise the tetrameric form of M2PYK. In the absence of amino-acid ligands an apparent tetramer-monomer dissociation is estimated to be ∼0.9 µM with a slow dissociation rate (∼15 min). X-ray structures of M2PYK complexes with alanine, phenylalanine, and tryptophan show the M2PYK locked in an inactive T-state conformation, while activators lock the M2PYK tetramer in the active R-state conformation. Amino-acid binding in the allosteric pocket triggers rigid body rotations (11°) stabilising either T or R states. The opposing inhibitory and activating effects of the non-essential amino acids serine and alanine suggest that M2PYK could act as a rapid-response nutrient sensor to rebalance cellular metabolism. This competition at a single allosteric site between activators and inhibitors provides a novel regulatory mechanism by which M2PYK activity is finely tuned by the relative (but not absolute) concentrations of activator and inhibitor amino acids. Such 'allostatic' regulation may be important in metabolic reprogramming and influencing cell fate.

PubMed: 29748232
DOI: 10.1042/BCJ20180171

実験手法

X-RAY DIFFRACTION (3.2 Å)