6GG0

Cryo-EM structure of BK polyomavirus like particle in complex with single chain antibody ScFv41F17

Summary for 6GG0

• Entry DOI: 10.2210/pdb6gg0/pdb
• EMDB information: 4398
• Descriptor: Capsid protein VP1, Heavy chain, light chain (3 entities in total)
• Functional Keywords: bk polyoma virus, single chain antibody, cross neutralizing antibody, virus like particle
• Biological source: BK polyomavirus (BKPyV); More
• Total number of polymer chains: 8
• Total formula weight: 266166.74

Authors

Srinivas, H. (deposition date: 2018-05-02, release date: 2019-03-06, Last modification date: 2025-07-09)

Primary citation

Lindner, J.M.,Cornacchione, V.,Sathe, A.,Be, C.,Srinivas, H.,Riquet, E.,Leber, X.C.,Hein, A.,Wrobel, M.B.,Scharenberg, M.,Pietzonka, T.,Wiesmann, C.,Abend, J.,Traggiai, E.
Human Memory B Cells Harbor Diverse Cross-Neutralizing Antibodies against BK and JC Polyomaviruses.
Immunity, 50:668-676.e5, 2019

PubMed Abstract: Human polyomaviruses cause a common childhood infection worldwide and typically elicit a neutralizing antibody and cellular immune response, while establishing a dormant infection in the kidney with minimal clinical manifestations. However, viral reactivation can cause severe pathology in immunocompromised individuals. We developed a high-throughput, functional antibody screen to examine the humoral response to BK polyomavirus. This approach enabled the isolation of antibodies from all peripheral B cell subsets and revealed the anti-BK virus antibody repertoire as clonally complex with respect to immunoglobulin sequences and isotypes (both IgM and IgG), including a high frequency of monoclonal antibodies that broadly neutralize BK virus subtypes and the related JC polyomavirus. Cryo-electron microscopy of a broadly neutralizing IgG single-chain variable fragment complexed with BK virus-like particles revealed the quaternary nature of a conserved viral epitope at the junction between capsid pentamers. These features unravel a potent modality for inhibiting polyomavirus infection in kidney transplant recipients and other immunocompromised patients.

PubMed: 30824324
DOI: 10.1016/j.immuni.2019.02.003

Experimental method

ELECTRON MICROSCOPY (4.24 Å)