6GEF

X-ray structure of the Yersinia pseudotuberculosis ATPase DotB

6GEF の概要

• エントリーDOI: 10.2210/pdb6gef/pdb
• 関連するPDBエントリー: 6GEB
• 分子名称: Type IV secretion system protein DotB (1 entity in total)
• 機能のキーワード: aaa+ atpase type ivb secretion, hydrolase
• 由来する生物種: Yersinia pseudotuberculosis IP 31758
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 270187.81

構造登録者

Prevost, M.S.,Waksman, G. (登録日: 2018-04-26, 公開日: 2018-05-30, 最終更新日: 2024-01-17)

主引用文献

Prevost, M.S.,Waksman, G.
X-ray crystal structures of the type IVb secretion system DotB ATPases.
Protein Sci., 27:1464-1475, 2018

PubMed Abstract: Human infections by the intracellular bacterial pathogen Legionella pneumophila result in a severe form of pneumonia, the Legionnaire's disease. L. pneumophila utilizes a Type IVb secretion (T4bS) system termed "dot/icm" to secrete protein effectors to the host cytoplasm. The dot/icm system is powered at least in part by a functionally critical AAA+ ATPase, a protein called DotB, thought to belong to the VirB11 family of proteins. Here we present the crystal structure of DotB at 3.19 Å resolution, in its hexameric form. We observe that DotB is in fact a structural intermediate between VirB11 and PilT family proteins, with a PAS-like N-terminal domain coupled to a RecA-like C-terminal domain. It also shares critical structural elements only found in PilT. The structure also reveals two conformers, termed α and β, with an αβαβαβ configuration. The existence of α and β conformers in this class of proteins was confirmed by solving the structure of DotB from another bacterial pathogen, Yersinia, where, intriguingly, we observed an ααβααβ configuration. The two conformers co-exist regardless of the nucleotide-bound states of the proteins. Our investigation therefore reveals that these ATPases can adopt a wider range of conformational states than was known before, shedding new light on the extraordinary spectrum of conformations these ATPases can access to carry out their function. Overall, the structure of DotB provides a template for further rational drug design to develop more specific antibiotics to tackle Legionnaire's disease. PDB Code(s): Will; be; provided.

PubMed: 29770512
DOI: 10.1002/pro.3439

実験手法

X-RAY DIFFRACTION (2.75 Å)