6GCZ

Laminarin binding SusD-like protein

6GCZ の概要

• エントリーDOI: 10.2210/pdb6gcz/pdb
• 分子名称: Starch-binding associating with outer membrane, GLYCEROL (3 entities in total)
• 機能のキーワード: laminarin, marine bacteria, x-ray crystal structure, sugar binding protein
• 由来する生物種: Gramella sp. MAR_2010_102
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 54800.42

構造登録者

Mystowka, A.,Robb, C.S.,Hehemann, J.H. (登録日: 2018-04-20, 公開日: 2018-10-17, 最終更新日: 2024-01-17)

主引用文献

Mystkowska, A.A.,Robb, C.,Vidal-Melgosa, S.,Vanni, C.,Fernandez-Guerra, A.,Hohne, M.,Hehemann, J.H.
Molecular recognition of the beta-glucans laminarin and pustulan by a SusD-like glycan-binding protein of a marine Bacteroidetes.
FEBS J., 285:4465-4481, 2018

PubMed Abstract: Marine bacteria catabolize carbohydrate polymers of algae, which synthesize these structurally diverse molecules in ocean surface waters. Although algal glycans are an abundant carbon and energy source in the ocean, the molecular details that enable specific recognition between algal glycans and bacterial degraders remain largely unknown. Here we characterized a surface protein, GMSusD from the planktonic Bacteroidetes-Gramella sp. MAR_2010_102 that thrives during algal blooms. Our biochemical and structural analyses show that GMSusD binds glucose polysaccharides such as branched laminarin and linear pustulan. The 1.8 Å crystal structure of GMSusD indicates that three tryptophan residues form the putative glycan-binding site. Mutagenesis studies confirmed that these residues are crucial for laminarin recognition. We queried metagenomes of global surface water datasets for the occurrence of SusD-like proteins and found sequences with the three structurally conserved residues in different locations in the ocean. The molecular selectivity of GMSusD underscores that specific interactions are required for laminarin recognition. In conclusion, our findings provide insight into the molecular details of β-glucan binding by GMSusD and our bioinformatic analysis reveals that this molecular interaction may contribute to glucan cycling in the surface ocean.

PubMed: 30300505
DOI: 10.1111/febs.14674

実験手法

X-RAY DIFFRACTION (1.8 Å)