6GC7

50S ribosomal subunit assembly intermediate state 1

6GC7 の概要

• エントリーDOI: 10.2210/pdb6gc7/pdb
• EMDBエントリー: 4378 4379 4380 4381 4382
• 分子名称: 23S ribosomal RNA, 50S ribosomal protein L21, 50S ribosomal protein L22, ... (17 entities in total)
• 機能のキーワード: assembly precursor 48s 50s biogenesis in vitro reconstitution, ribosome
• 由来する生物種: Escherichia coli K-12; 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 1141442.99

構造登録者

Nikolay, R.,Hilal, T.,Qin, B.,Loerke, J.,Buerger, J.,Mielke, T.,Spahn, C.M.T. (登録日: 2018-04-17, 公開日: 2018-06-20, 最終更新日: 2024-05-15)

主引用文献

Nikolay, R.,Hilal, T.,Qin, B.,Mielke, T.,Burger, J.,Loerke, J.,Textoris-Taube, K.,Nierhaus, K.H.,Spahn, C.M.T.
Structural Visualization of the Formation and Activation of the 50S Ribosomal Subunit during In Vitro Reconstitution.
Mol. Cell, 70:881-893.e3, 2018

PubMed Abstract: The assembly of ribosomal subunits is an essential prerequisite for protein biosynthesis in all domains of life. Although biochemical and biophysical approaches have advanced our understanding of ribosome assembly, our mechanistic comprehension of this process is still limited. Here, we perform an in vitro reconstitution of the Escherichia coli 50S ribosomal subunit. Late reconstitution products were subjected to high-resolution cryo-electron microscopy and multiparticle refinement analysis to reconstruct five distinct precursors of the 50S subunit with 4.3-3.8 Å resolution. These assembly intermediates define a progressive maturation pathway culminating in a late assembly particle, whose structure is more than 96% identical to a mature 50S subunit. Our structures monitor the formation and stabilization of structural elements in a nascent particle in unprecedented detail and identify the maturation of the rRNA-based peptidyl transferase center as the final critical step along the 50S assembly pathway.

PubMed: 29883607
DOI: 10.1016/j.molcel.2018.05.003

実験手法

ELECTRON MICROSCOPY (4.3 Å)