6GBY

Copper nitrite reductase from Achromobacter cycloclastes: non-polymorph separated dataset 1

6GBY の概要

• エントリーDOI: 10.2210/pdb6gby/pdb
• 分子名称: Copper-containing nitrite reductase, COPPER (II) ION, NITRITE ION, ... (4 entities in total)
• 機能のキーワード: copper, denitrification, serial synchrotron crystallography, polymorphs, nitrite reductase, oxidoreductase
• 由来する生物種: Achromobacter cycloclastes
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40992.27

構造登録者

Ebrahim, A.,Appleby, M.V.,Axford, D.,Beale, J.,Moreno-Chicano, T.,Sherrell, D.A.,Strange, R.W.,Owen, R.L.,Hough, M.A. (登録日: 2018-04-16, 公開日: 2019-01-30, 最終更新日: 2024-01-17)

主引用文献

Ebrahim, A.,Appleby, M.V.,Axford, D.,Beale, J.,Moreno-Chicano, T.,Sherrell, D.A.,Strange, R.W.,Hough, M.A.,Owen, R.L.
Resolving polymorphs and radiation-driven effects in microcrystals using fixed-target serial synchrotron crystallography.
Acta Crystallogr D Struct Biol, 75:151-159, 2019

PubMed Abstract: The ability to determine high-quality, artefact-free structures is a challenge in micro-crystallography, and the rapid onset of radiation damage and requirement for a high-brilliance X-ray beam mean that a multi-crystal approach is essential. However, the combination of crystal-to-crystal variation and X-ray-induced changes can make the formation of a final complete data set challenging; this is particularly true in the case of metalloproteins, where X-ray-induced changes occur rapidly and at the active site. An approach is described that allows the resolution, separation and structure determination of crystal polymorphs, and the tracking of radiation damage in microcrystals. Within the microcrystal population of copper nitrite reductase, two polymorphs with different unit-cell sizes were successfully separated to determine two independent structures, and an X-ray-driven change between these polymorphs was followed. This was achieved through the determination of multiple serial structures from microcrystals using a high-throughput high-speed fixed-target approach coupled with robust data processing.

PubMed: 30821704
DOI: 10.1107/S2059798318010240

実験手法

X-RAY DIFFRACTION (1.48 Å)