6G9C

Crystal structure of immunomodulatory active chitinase from Trichuris suis, TsES1

6G9C の概要

• エントリーDOI: 10.2210/pdb6g9c/pdb
• 分子名称: Immunomodulatory active chitinase, 1,2-ETHANEDIOL (3 entities in total)
• 機能のキーワード: tses1, immunomodulatory potential, chitinase, eukaryotic protein expression, lexsy, secretion, dimer, intramolecular disulphide bridge, trichuris suis, hydrolase
• 由来する生物種: Trichuris suis (pig whipworm)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 111774.13

構造登録者

Malecki, P.H.,Balster, K.,Hartmann, S.,Weiss, M.S.,Heinemann, U. (登録日: 2018-04-10, 公開日: 2019-04-24, 最終更新日: 2024-01-31)

主引用文献

Ebner, F.,Lindner, K.,Janek, K.,Niewienda, A.,Malecki, P.H.,Weiss, M.S.,Sutherland, T.E.,Heuser, A.,Kuhl, A.A.,Zentek, J.,Hofmann, A.,Hartmann, S.
A Helminth-Derived Chitinase Structurally Similar to Mammalian Chitinase Displays Immunomodulatory Properties in Inflammatory Lung Disease.
J Immunol Res, 2021:6234836-6234836, 2021

PubMed Abstract: Immunomodulation of airway hyperreactivity by excretory-secretory (ES) products of the first larval stage (L1) of the gastrointestinal nematode is reported by us and others. Here, we aimed to identify the proteins accounting for the modulatory effects of the L1 ES proteins and studied six selected L1 proteins for their immunomodulatory efficacy in a murine OVA-induced allergic airway disease model. In particular, an enzymatically active chitinase mediated amelioration of clinical signs of airway hyperreactivity, primarily associated with suppression of eosinophil recruitment into the lung, the associated chemokines, and increased numbers of RELM interstitial lung macrophages. While there is no indication of chitinase directly interfering with dendritic cell activation or antigen presentation to CD4 T cells, treatment of allergic mice with the worm chitinase influenced the hosts' own chitinase activity in the inflamed lung. The three-dimensional structure of the chitinase as determined by high-resolution X-ray crystallography revealed high similarities to mouse acidic mammalian chitinase (AMCase) but a unique ability of chitinase to form dimers. Our data indicate that the structural similarities between the parasite and host chitinase contribute to the disease-ameliorating effect of the helminth-derived chitinase on allergic lung inflammation.

PubMed: 34869783
DOI: 10.1155/2021/6234836

実験手法

X-RAY DIFFRACTION (1.74 Å)