6G7C

Nt2-CTD domains of the TssA component from the type VI secretion system of Aeromonas hydrophila.

6G7C の概要

• エントリーDOI: 10.2210/pdb6g7c/pdb
• 分子名称: ImpA-related domain protein (1 entity in total)
• 機能のキーワード: type vi secretion system component, alpha helical protein, tssa, aeromonas hydrophila, transport protein
• 由来する生物種: Aeromonas hydrophila subsp. hydrophila ATCC 7966
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 295903.73

構造登録者

Dix, S.D.,Owen, H.J.,Sun, R.,Ahmad, A.,Shastri, S.,Spiewak, H.L.,Mosby, D.J.,Harris, M.J.,Batters, S.L.,Tzokov, S.B.,Sedelnikova, S.E.,Baker, P.J.,Bullough, P.A.,Rice, D.W.,Thomas, M.S. (登録日: 2018-04-05, 公開日: 2018-11-21, 最終更新日: 2024-05-08)

主引用文献

Dix, S.R.,Owen, H.J.,Sun, R.,Ahmad, A.,Shastri, S.,Spiewak, H.L.,Mosby, D.J.,Harris, M.J.,Batters, S.L.,Brooker, T.A.,Tzokov, S.B.,Sedelnikova, S.E.,Baker, P.J.,Bullough, P.A.,Rice, D.W.,Thomas, M.S.
Structural insights into the function of type VI secretion system TssA subunits.
Nat Commun, 9:4765-4765, 2018

PubMed Abstract: The type VI secretion system (T6SS) is a multi-protein complex that injects bacterial effector proteins into target cells. It is composed of a cell membrane complex anchored to a contractile bacteriophage tail-like apparatus consisting of a sharpened tube that is ejected by the contraction of a sheath against a baseplate. We present structural and biochemical studies on TssA subunits from two different T6SSs that reveal radically different quaternary structures in comparison to the dodecameric E. coli TssA that arise from differences in their C-terminal sequences. Despite this, the different TssAs retain equivalent interactions with other components of the complex and position their highly conserved N-terminal ImpA_N domain at the same radius from the centre of the sheath as a result of their distinct domain architectures, which includes additional spacer domains and highly mobile interdomain linkers. Together, these variations allow these distinct TssAs to perform a similar function in the complex.

PubMed: 30420757
DOI: 10.1038/s41467-018-07247-1

実験手法

X-RAY DIFFRACTION (3.13 Å)