6G72

Mouse mitochondrial complex I in the deactive state

Summary for 6G72

• Entry DOI: 10.2210/pdb6g72/pdb
• Related: 6G2J
• EMDB information: 4345 4356
• Descriptor: NADH-ubiquinone oxidoreductase chain 3, NADH-ubiquinone oxidoreductase chain 6, NADH-ubiquinone oxidoreductase chain 4L, ... (52 entities in total)
• Functional Keywords: complex i, mitochondria, proton pump, membrane protein, oxidoreductase
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 45
• Total formula weight: 1052147.47

Authors

Agip, A.N.A.,Blaza, J.N.,Bridges, H.R.,Viscomi, C.,Rawson, S.,Muench, S.P.,Hirst, J. (deposition date: 2018-04-04, release date: 2018-06-06, Last modification date: 2024-05-29)

Primary citation

Agip, A.A.,Blaza, J.N.,Bridges, H.R.,Viscomi, C.,Rawson, S.,Muench, S.P.,Hirst, J.
Cryo-EM structures of complex I from mouse heart mitochondria in two biochemically defined states.
Nat. Struct. Mol. Biol., 25:548-556, 2018

PubMed Abstract: Complex I (NADH:ubiquinone oxidoreductase) uses the reducing potential of NADH to drive protons across the energy-transducing inner membrane and power oxidative phosphorylation in mammalian mitochondria. Recent cryo-EM analyses have produced near-complete models of all 45 subunits in the bovine, ovine and porcine complexes and have identified two states relevant to complex I in ischemia-reperfusion injury. Here, we describe the 3.3-Å structure of complex I from mouse heart mitochondria, a biomedically relevant model system, in the 'active' state. We reveal a nucleotide bound in subunit NDUFA10, a nucleoside kinase homolog, and define mechanistically critical elements in the mammalian enzyme. By comparisons with a 3.9-Å structure of the 'deactive' state and with known bacterial structures, we identify differences in helical geometry in the membrane domain that occur upon activation or that alter the positions of catalytically important charged residues. Our results demonstrate the capability of cryo-EM analyses to challenge and develop mechanistic models for mammalian complex I.

PubMed: 29915388
DOI: 10.1038/s41594-018-0073-1

Experimental method

ELECTRON MICROSCOPY (3.9 Å)