6G4K

The solution NMR structure of brevinin-1BYa in sodium dodecyl sulphate micelles

6G4K の概要

• エントリーDOI: 10.2210/pdb6g4k/pdb
• NMR情報: BMRB: 34251
• 分子名称: Brevinin-1BYa (1 entity in total)
• 機能のキーワード: cationic, rana-box, antimicrobial peptide, antimicrobial protein
• 由来する生物種: Rana boylii (Foothill yellow-legged frog)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2613.32

構造登録者

Timmons, P.B.,O'Flynn, D.P.,Conlon, J.M.,Hewage, C.M. (登録日: 2018-03-27, 公開日: 2019-10-09, 最終更新日: 2023-06-14)

主引用文献

Timmons, P.B.,O'Flynn, D.,Conlon, J.M.,Hewage, C.M.
Structural and positional studies of the antimicrobial peptide brevinin-1BYa in membrane-mimetic environments.
J.Pept.Sci., 25:e3208-e3208, 2019

PubMed Abstract: Brevinin-1BYa (FLPILASLAAKFGPKLFCLVTKKC), first isolated from skin secretions of the foothill yellow-legged frog Rana boylii, shows broad-spectrum activity, being particularly effective against opportunistic yeast pathogens. The structure of brevinin-1BYa was investigated in various solution and membrane-mimicking environments by proton nuclear magnetic resonance ( H-NMR) spectroscopy and molecular modelling. The peptide does not possess a secondary structure in aqueous solution. In a 33% 2,2,2-trifluoroethanol (TFE-d )-H O solvent mixture, as well as in membrane-mimicking sodium dodecyl sulfate and dodecylphosphocholine micelles, the peptide's structure is characterised by a flexible helix-hinge-helix motif, with the hinge located at the Gly /Pro residues, and the two α-helices extending from Pro to Phe and from Pro to Thr . Positional studies involving the peptide in sodium dodecyl sulfate and dodecylphosphocholine micelles using 5-doxyl-labelled stearic acid and manganese chloride paramagnetic probes show that the peptide's helical segments lie parallel to the micellar surface, with the residues on the hydrophobic face of the amphipathic helices facing towards the micelle core and the hydrophilic residues pointing outwards, suggesting that the peptide exerts its biological activity by a non-pore-forming mechanism.

PubMed: 31721374
DOI: 10.1002/psc.3208

実験手法

SOLUTION NMR