6G3S

Structure of tellurium-centred Anderson-Evans polyoxotungstate (TEW) bound to the nucleotide binding domain of HSP70. Second structure of two TEW-HSP70 structures deposited.

6G3S の概要

• エントリーDOI: 10.2210/pdb6g3s/pdb
• 分子名称: Heat shock 70 kDa protein 1A, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (7 entities in total)
• 機能のキーワード: atpase domain, chaperone
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45727.09

構造登録者

Mac Sweeney, A.,Chambovey, A.,Wicki, M.,Mueller, M.,Artico, N.,Lange, R.,Bijelic, A.,Breibeck, J.,Rompel, A. (登録日: 2018-03-26, 公開日: 2018-10-17, 最終更新日: 2024-01-17)

主引用文献

Mac Sweeney, A.,Chambovey, A.,Wicki, M.,Muller, M.,Artico, N.,Lange, R.,Bijelic, A.,Breibeck, J.,Rompel, A.
The crystallization additive hexatungstotellurate promotes the crystallization of the HSP70 nucleotide binding domain into two different crystal forms.
PLoS ONE, 13:e0199639-e0199639, 2018

PubMed Abstract: The use of the tellurium-centered Anderson-Evans polyoxotungstate [TeW6O24]6- (TEW) as a crystallization additive has been described. Here, we present the use of TEW as an additive in the crystallization screening of the nucleotide binding domain (NBD) of HSP70. Crystallization screening of the HSP70 NBD in the absence of TEW using a standard commercial screen resulted in a single crystal form. An identical crystallization screen of the HSP70 NBD in the presence of TEW resulted in both the "TEW free" crystal form and an additional crystal form with a different crystal packing. TEW binding was observed in both crystal forms, either as a well-defined molecule or in overlapping alternate positions suggesting translational disorder. The structures were solved by both molecular replacement and single wavelength anomalous diffraction (SAD) using the anomalous signal of a single bound molecule of TEW. This study adds one more example of TEW binding to a protein and influencing its crystallization behavior.

PubMed: 29949628
DOI: 10.1371/journal.pone.0199639

実験手法

X-RAY DIFFRACTION (2.3 Å)