6G20

Crystal structure of a fluorescence optimized bathy phytochrome PAiRFP2 derived from wild-type Agp2 in its functional Meta-F intermediate state.

Summary for 6G20

Related6G1Y 6G1Z
DescriptorBacteriophytochrome protein, Biliverdin, SULFATE ION, ... (9 entities in total)
Functional Keywordsfluorescent protein, signaling protein, phytochrome, bacteriophytochrome, bilin chromophore, photoisomerization, signal transduction, meta-f state, light adaptation, bathy phytochrome, photosensory core module, optogenetic, flourescence
Biological sourceAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Total number of polymer chains2
Total molecular weight117714
Authors
Schmidt, A.,Sauthof, L.,Szczepek, M.,Scheerer, P. (deposition date: 2018-03-22, release date: 2018-11-28, Last modification date: 2018-12-05)
Primary citation
Schmidt, A.,Sauthof, L.,Szczepek, M.,Lopez, M.F.,Escobar, F.V.,Qureshi, B.M.,Michael, N.,Buhrke, D.,Stevens, T.,Kwiatkowski, D.,von Stetten, D.,Mroginski, M.A.,Krauss, N.,Lamparter, T.,Hildebrandt, P.,Scheerer, P.
Structural snapshot of a bacterial phytochrome in its functional intermediate state.
Nat Commun, 9:4912-4912, 2018
PubMed: 30464203 (PDB entries with the same primary citation)
DOI: 10.1038/s41467-018-07392-7
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.16 Å)
?

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.23620 0.3% 2.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 6g20
no rotation
Molmil generated image of 6g20
rotated about x axis by 90°
Molmil generated image of 6g20
rotated about y axis by 90°

More Biological unit images

Molmil generated image of 6g20
no rotation
Molmil generated image of 6g20
rotated about x axis by 90°
Molmil generated image of 6g20
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (6g20.pdb2.gz [1000.17 KB])
Coordinate files for Biological unit (6g20.pdb1.gz [969.38 KB])