6G20

Crystal structure of a fluorescence optimized bathy phytochrome PAiRFP2 derived from wild-type Agp2 in its functional Meta-F intermediate state.

Summary for 6G20

• Entry DOI: 10.2210/pdb6g20/pdb
• Related: 6G1Y 6G1Z
• Descriptor: Bacteriophytochrome protein, 3-[(2Z)-2-({3-(2-carboxyethyl)-5-[(E)-(4-ethenyl-3-methyl-5-oxo-1,5-dihydro-2H-pyrrol-2-ylidene)methyl]-4-methyl-1H-pyrrol-2-yl}methylidene)-5-{(Z)-[(3E,4S)-3-ethylidene-4-methyl-5-oxopyrrolidin-2-ylidene]methyl}-4-methyl-2H-pyrrol-3-yl]propanoic acid, SULFATE ION, ... (9 entities in total)
• Functional Keywords: fluorescent protein, signaling protein, phytochrome, bacteriophytochrome, bilin chromophore, photoisomerization, signal transduction, meta-f state, light adaptation, bathy phytochrome, photosensory core module, optogenetic, flourescence
• Biological source: Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
• Total number of polymer chains: 2
• Total formula weight: 117714.00

Authors

Schmidt, A.,Sauthof, L.,Szczepek, M.,Scheerer, P. (deposition date: 2018-03-22, release date: 2018-11-28, Last modification date: 2024-02-07)

• Primary citation: Schmidt, A.,Sauthof, L.,Szczepek, M.,Lopez, M.F.,Escobar, F.V.,Qureshi, B.M.,Michael, N.,Buhrke, D.,Stevens, T.,Kwiatkowski, D.,von Stetten, D.,Mroginski, M.A.,Krauss, N.,Lamparter, T.,Hildebrandt, P.,Scheerer, P.; Structural snapshot of a bacterial phytochrome in its functional intermediate state.; Nat Commun, 9:4912-4912, 2018; PubMed: 30464203; DOI: 10.1038/s41467-018-07392-7

Experimental method

X-RAY DIFFRACTION (2.16 Å)