6G18

Cryo-EM structure of a late human pre-40S ribosomal subunit - State C

6G18 の概要

• エントリーDOI: 10.2210/pdb6g18/pdb
• EMDBエントリー: 4337
• 分子名称: pre-18S ribosomal RNA, 40S ribosomal protein S28, Ubiquitin-40S ribosomal protein S27a, ... (36 entities in total)
• 機能のキーワード: 40s, pre-40s, ribosome biogenesis, ribosome
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 35
• 化学式量合計: 1479288.86

構造登録者

Ameismeier, M.,Cheng, J.,Berninghausen, O.,Beckmann, R. (登録日: 2018-03-20, 公開日: 2018-06-06, 最終更新日: 2024-05-15)

主引用文献

Ameismeier, M.,Cheng, J.,Berninghausen, O.,Beckmann, R.
Visualizing late states of human 40S ribosomal subunit maturation.
Nature, 558:249-253, 2018

PubMed Abstract: The formation of eukaryotic ribosomal subunits extends from the nucleolus to the cytoplasm and entails hundreds of assembly factors. Despite differences in the pathways of ribosome formation, high-resolution structural information has been available only from fungi. Here we present cryo-electron microscopy structures of late-stage human 40S assembly intermediates, representing one state reconstituted in vitro and five native states that range from nuclear to late cytoplasmic. The earliest particles reveal the position of the biogenesis factor RRP12 and distinct immature rRNA conformations that accompany the formation of the 40S subunit head. Molecular models of the late-acting assembly factors TSR1, RIOK1, RIOK2, ENP1, LTV1, PNO1 and NOB1 provide mechanistic details that underlie their contribution to a sequential 40S subunit assembly. The NOB1 architecture displays an inactive nuclease conformation that requires rearrangement of the PNO1-bound 3' rRNA, thereby coordinating the final rRNA folding steps with site 3 cleavage.

PubMed: 29875412
DOI: 10.1038/s41586-018-0193-0

実験手法

ELECTRON MICROSCOPY (3.6 Å)