6G0J

Inactive Fe-PP1

6G0J の概要

• エントリーDOI: 10.2210/pdb6g0j/pdb
• 分子名称: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, FE (III) ION, MANGANESE (II) ION, ... (5 entities in total)
• 機能のキーワード: phosphatase, iron, oxidative stress, cell cycle, hydrolase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37963.64

構造登録者

Salvi, F.,Barabas, O.,Koehn, M. (登録日: 2018-03-18, 公開日: 2018-11-21, 最終更新日: 2024-11-13)

主引用文献

Salvi, F.,Trebacz, M.,Kokot, T.,Hoermann, B.,Rios, P.,Barabas, O.,Koehn, M.
Effects of stably incorporated iron on protein phosphatase-1 structure and activity.
FEBS Lett., 592:4028-4038, 2018

PubMed Abstract: Protein phosphatase-1 (PP1) drives a large amount of phosphoSer/Thr protein dephosphorylations in eukaryotes to counteract multiple kinases in signaling pathways. The phosphatase requires divalent metal cations for catalytic activity and contains iron naturally. Iron has been suggested to have an influence on PP1 activity through Fe and Fe oxidation states. However, much biochemical and all structural data have been obtained with recombinant PP1 containing Mn ions. Purifying iron-containing PP1 from Escherichia coli has thus far not been possible. Here, we present the preparation, characterization, and structure of iron-bound PP1α in inactive and active states. We establish a key role for the electronic/redox properties of iron in PP1 activity and shed light on the difference in substrate specificity between iron- and manganese-containing PP1.

PubMed: 30403291
DOI: 10.1002/1873-3468.13284

実験手法

X-RAY DIFFRACTION (2.1 Å)