6FXB

Bovine beta-lactoglobulin variant A at pH 4.0

6FXB の概要

• エントリーDOI: 10.2210/pdb6fxb/pdb
• 分子名称: Major allergen beta-lactoglobulin, NITRATE ION, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: lactoglobulin, milk protein, allergen
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 73779.19

構造登録者

Khan, S.,Ipsen, R.,Almdal, K.,Svensson, B.,Harris, P. (登録日: 2018-03-08, 公開日: 2018-05-23, 最終更新日: 2024-10-23)

主引用文献

Khan, S.,Ipsen, R.,Almdal, K.,Svensson, B.,Harris, P.
Revealing the Dimeric Crystal and Solution Structure of beta-Lactoglobulin at pH 4 and Its pH and Salt Dependent Monomer-Dimer Equilibrium.
Biomacromolecules, 19:2905-2912, 2018

PubMed Abstract: The dimeric structure of bovine β-lactoglobulin A (BLGA) at pH 4.0 was solved to 2.0 Å resolution. Fitting the BLGA pH 4.0 structure to SAXS data at low ionic strength (goodness of fit R-factor = 3.6%) verified the dimeric state in solution. Analysis of the monomer-dimer equilibrium at varying pH and ionic strength by SAXS and scattering modeling showed that BLGA is dimeric at pH 3.0 and 4.0, shifting toward a monomer at pH 2.2, 2.6, and 7.0 yielding monomer/dimer ratios of 80/20%, 50/50%, and 25/75%, respectively. BLGA remained a dimer at pH 3.0 and 4.0 in 50-150 mM NaCl, whereas the electrostatic shielding raised the dimer content at pH 2.2, 2.6, and 7.0, i.e., below and above the pI. Overall, the findings provide new insights into the molecular characteristics of BLGA relevant for dairy product formulations and for various biotechnological and pharmaceutical applications.

PubMed: 29737841
DOI: 10.1021/acs.biomac.8b00471

実験手法

X-RAY DIFFRACTION (2 Å)