6FVH

Macrophage Migration Inhibitory Factor (MIF) with Covalently Bound PITC

6FVH の概要

• エントリーDOI: 10.2210/pdb6fvh/pdb
• 分子名称: Macrophage migration inhibitory factor, SULFATE ION, N-phenylthioformamide, ... (4 entities in total)
• 機能のキーワード: isomerase inhibitor, isomerase
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 38053.15

構造登録者

Samygina, V.R.,Bourenkov, G.,Sokolov, A.V. (登録日: 2018-03-02, 公開日: 2018-06-06, 最終更新日: 2024-11-13)

主引用文献

Sokolov, A.V.,Dadinova, L.A.,Petoukhov, M.V.,Bourenkov, G.,Dubova, K.M.,Amarantov, S.V.,Volkov, V.V.,Kostevich, V.A.,Gorbunov, N.P.,Grudinina, N.A.,Vasilyev, V.B.,Samygina, V.R.
Structural Study of the Complex Formed by Ceruloplasmin and Macrophage Migration Inhibitory Factor.
Biochemistry Mosc., 83:701-707, 2018

PubMed Abstract: Macrophage migration inhibitory factor (MIF) is a key proinflammatory cytokine. Inhibitors of tautomerase activity of MIF are perspective antiinflammatory compounds. Ceruloplasmin, the copper-containing ferroxidase of blood plasma, is a noncompetitive inhibitor of tautomerase activity of MIF in the reaction with p-hydroxyphenylpyruvate. Small-angle X-ray scattering established a model of the complex formed by MIF and ceruloplasmin. Crystallographic analysis of MIF with a modified active site supports the model. The stoichiometry of 3 CP/MIF trimer complex was established using gel filtration. Conformity of novel data concerning the interaction regions in the studied proteins with previous biochemical data is discussed.

PubMed: 30195326
DOI: 10.1134/S000629791806007X

実験手法

X-RAY DIFFRACTION (1.4 Å)