6FS7

Influenza A/California/04/2009 (pH1N1) endonuclease with I38T mutation with bound inhibitor, baloxavir acid (BXA)

6FS7 の概要

• エントリーDOI: 10.2210/pdb6fs7/pdb
• 分子名称: Polymerase acidic protein,Polymerase acidic protein, MANGANESE (II) ION, Baloxavir acid, ... (4 entities in total)
• 機能のキーワード: influenza, endonuclease, inhibitor, viral protein
• 由来する生物種: Influenza A virus (A/California/04/2009(H1N1)); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 138642.16

構造登録者

Cusack, S.,Speranzini, V. (登録日: 2018-02-19, 公開日: 2018-07-11, 最終更新日: 2024-01-17)

主引用文献

Omoto, S.,Speranzini, V.,Hashimoto, T.,Noshi, T.,Yamaguchi, H.,Kawai, M.,Kawaguchi, K.,Uehara, T.,Shishido, T.,Naito, A.,Cusack, S.
Characterization of influenza virus variants induced by treatment with the endonuclease inhibitor baloxavir marboxil.
Sci Rep, 8:9633-9633, 2018

PubMed Abstract: Baloxavir acid (BXA), derived from the prodrug baloxavir marboxil (BXM), potently and selectively inhibits the cap-dependent endonuclease within the polymerase PA subunit of influenza A and B viruses. In clinical trials, single doses of BXM profoundly decrease viral titers as well as alleviating influenza symptoms. Here, we characterize the impact on BXA susceptibility and replicative capacity of variant viruses detected in the post-treatment monitoring of the clinical studies. We find that the PA I38T substitution is a major pathway for reduced susceptibility to BXA, with 30- to 50-fold and 7-fold EC changes in A and B viruses, respectively. The viruses harboring the I38T substitution show severely impaired replicative fitness in cells, and correspondingly reduced endonuclease activity in vitro. Co-crystal structures of wild-type and I38T influenza A and B endonucleases bound to BXA show that the mutation reduces van der Waals contacts with the inhibitor. A reduced affinity to the I38T mutant is supported by the lower stability of the BXA-bound endonuclease. These mechanistic insights provide markers for future surveillance of treated populations.

PubMed: 29941893
DOI: 10.1038/s41598-018-27890-4

実験手法

X-RAY DIFFRACTION (1.96 Å)