6FRJ

Crystal structure of scFv-SM3 in complex with APD-SeThrGalNAc-RP

6FRJ の概要

• エントリーDOI: 10.2210/pdb6frj/pdb
• 分子名称: scFv-SM3, APD-SeThr-RP, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: immune system, glycopeptides, antibodies, molecular recognition, conformation analysis
• 由来する生物種: Mus musculus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26945.49

構造登録者

Companon, I.,Castro-Lopez, J.,Escudero-Casao, M.,Avenoza, A.,Busto, J.H.,Castillon, S.,Jimenez-Barbero, J.,Bernardes, G.J.,Boutureira, O.,Jimenez-Oses, G.,Asensio, J.L.,Peregrina, J.M.,Hurtado-Guerrero, R.,Corzana, F. (登録日: 2018-02-16, 公開日: 2019-02-20, 最終更新日: 2024-11-06)

主引用文献

Companon, I.,Guerreiro, A.,Mangini, V.,Castro-Lopez, J.,Escudero-Casao, M.,Avenoza, A.,Busto, J.H.,Castillon, S.,Jimenez-Barbero, J.,Asensio, J.L.,Jimenez-Oses, G.,Boutureira, O.,Peregrina, J.M.,Hurtado-Guerrero, R.,Fiammengo, R.,Bernardes, G.J.L.,Corzana, F.
Structure-Based Design of Potent Tumor-Associated Antigens: Modulation of Peptide Presentation by Single-Atom O/S or O/Se Substitutions at the Glycosidic Linkage.
J. Am. Chem. Soc., 141:4063-4072, 2019

PubMed Abstract: GalNAc-glycopeptides derived from mucin MUC1 are an important class of tumor-associated antigens. α- O-glycosylation forces the peptide to adopt an extended conformation in solution, which is far from the structure observed in complexes with a model anti-MUC1 antibody. Herein, we propose a new strategy for designing potent antigen mimics based on modulating peptide/carbohydrate interactions by means of O → S/Se replacement at the glycosidic linkage. These minimal chemical modifications bring about two key structural changes to the glycopeptide. They increase the carbohydrate-peptide distance and change the orientation and dynamics of the glycosidic linkage. As a result, the peptide acquires a preorganized and optimal structure suited for antibody binding. Accordingly, these new glycopeptides display improved binding toward a representative anti-MUC1 antibody relative to the native antigens. To prove the potential of these glycopeptides as tumor-associated MUC1 antigen mimics, the derivative bearing the S-glycosidic linkage was conjugated to gold nanoparticles and tested as an immunogenic formulation in mice without any adjuvant, which resulted in a significant humoral immune response. Importantly, the mice antisera recognize cancer cells in biopsies of breast cancer patients with high selectivity. This finding demonstrates that the antibodies elicited against the mimetic antigen indeed recognize the naturally occurring antigen in its physiological context. Clinically, the exploitation of tumor-associated antigen mimics may contribute to the development of cancer vaccines and to the improvement of cancer diagnosis based on anti-MUC1 antibodies. The methodology presented here is of general interest for applications because it may be extended to modulate the affinity of biologically relevant glycopeptides toward their receptors.

PubMed: 30726084
DOI: 10.1021/jacs.8b13503

実験手法

X-RAY DIFFRACTION (1.4 Å)