6FQ2

Structure of minimal sequence for left -handed G-quadruplex formation

Summary for 6FQ2

• Entry DOI: 10.2210/pdb6fq2/pdb
• Related: 4U5M
• Descriptor: DNA (5'-D(*GP*TP*GP*GP*TP*GP*GP*TP*GP*GP*TP*G)-3'), POTASSIUM ION (3 entities in total)
• Functional Keywords: g-quadruplex, dna
• Biological source: synthetic construct
• Total number of polymer chains: 2
• Total formula weight: 7728.21

Authors

Schmitt, E.,Mechulam, Y.,Phan, A.T.,Heddi, B.,Bakalar, B. (deposition date: 2018-02-13, release date: 2018-12-05, Last modification date: 2024-01-17)

Primary citation

Bakalar, B.,Heddi, B.,Schmitt, E.,Mechulam, Y.,Phan, A.T.
A Minimal Sequence for Left-Handed G-Quadruplex Formation.
Angew. Chem. Int. Ed. Engl., 58:2331-2335, 2019

PubMed Abstract: Recently, we observed the first example of a left-handed G-quadruplex structure formed by natural DNA, named Z-G4. We analysed the Z-G4 structure and inspected its primary 28-nt sequence in order to identify motifs that convey the unique left-handed twist. Using circular dichroism spectroscopy, NMR spectroscopy, and X-ray crystallography, we revealed a minimal sequence motif of 12 nt (GTGGTGGTGGTG) for formation of the left-handed DNA G-quadruplex, which is found to be highly abundant in the human genome. A systematic analysis of thymine loop mutations revealed a moderate sequence tolerance, which would further broaden the space of sequences prone to left-handed G-quadruplex formation.

PubMed: 30481397
DOI: 10.1002/anie.201812628

Experimental method

X-RAY DIFFRACTION (2.31 Å)