6FOO

Structure of Ryanodine Receptor 1 in nanodiscs in the presence of calcium and ATP

これはPDB形式変換不可エントリーです。

6FOO の概要

• エントリーDOI: 10.2210/pdb6foo/pdb
• EMDBエントリー: 4258 4295
• 分子名称: Ryanodine receptor 1, ZINC ION (2 entities in total)
• 機能のキーワード: calcium release channel, nanodiscs, membrane protein
• 由来する生物種: Oryctolagus cuniculus (Rabbit); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 2131100.39

構造登録者

Willegems, K.,Efremov, R.G. (登録日: 2018-02-08, 公開日: 2018-08-08, 最終更新日: 2024-05-15)

主引用文献

Willegems, K.,Efremov, R.G.
Influence of Lipid Mimetics on Gating of Ryanodine Receptor.
Structure, 26:1303-1313.e4, 2018

PubMed Abstract: Understanding gating principles of ion channels at high resolution is of great importance. Here we investigate the conformational transition from closed to open state in ryanodine receptor 1 (RyR1) reconstituted into lipid nanodiscs. RyR1 is a homotetrameric giant ion channel that couples excitation of muscle cells to fast calcium release from the sarcoplasmic reticulum. Using single-particle cryo-EM we show that RyR1 reconstituted into lipid nanodiscs is stabilized in the open conformation when bound to the plant toxin ryanodine, but not in the presence of its physiological activators, calcium and ATP. Further, using ryanodine binding assays we show that membrane mimetics influence RyR1 transition between closed and open-channel conformations. We find that all detergents, including fluorinated detergents added to nanodiscs, stabilize closed state of RyR1. Our biochemical results correlate with available structural data and suggest optimal conditions for structural studies of RyR1 gating.

PubMed: 30078641
DOI: 10.1016/j.str.2018.06.010

実験手法

ELECTRON MICROSCOPY (8.2 Å)