6FJ2

Structure of Thermolysin solved from SAD data collected at the peak of the Zn absorption edge on ID30B

Summary for 6FJ2

• Entry DOI: 10.2210/pdb6fj2/pdb
• Related: 6fid
• Descriptor: Thermolysin, ZINC ION, CALCIUM ION, ... (8 entities in total)
• Functional Keywords: native, hydrolase
• Biological source: Bacillus thermoproteolyticus
• Total number of polymer chains: 1
• Total formula weight: 35228.97

Authors

McCarthy, A.A.,Mueller-Dieckmann, C. (deposition date: 2018-01-19, release date: 2018-01-31, Last modification date: 2024-05-08)

Primary citation

McCarthy, A.A.,Barrett, R.,Beteva, A.,Caserotto, H.,Dobias, F.,Felisaz, F.,Giraud, T.,Guijarro, M.,Janocha, R.,Khadrouche, A.,Lentini, M.,Leonard, G.A.,Lopez Marrero, M.,Malbet-Monaco, S.,McSweeney, S.,Nurizzo, D.,Papp, G.,Rossi, C.,Sinoir, J.,Sorez, C.,Surr, J.,Svensson, O.,Zander, U.,Cipriani, F.,Theveneau, P.,Mueller-Dieckmann, C.
ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF.
J Synchrotron Radiat, 25:1249-1260, 2018

PubMed Abstract: ID30B is an undulator-based high-intensity, energy-tuneable (6.0-20 keV) and variable-focus (20-200 µm in diameter) macromolecular crystallography (MX) beamline at the ESRF. It was the last of the ESRF Structural Biology Group's beamlines to be constructed and commissioned as part of the ESRF's Phase I Upgrade Program and has been in user operation since June 2015. Both a modified microdiffractometer (MD2S) incorporating an in situ plate screening capability and a new flexible sample changer (the FlexHCD) were specifically developed for ID30B. Here, the authors provide the current beamline characteristics and detail how different types of MX experiments can be performed on ID30B (http://www.esrf.eu/id30b).

PubMed: 29979188
DOI: 10.1107/S1600577518007166

Experimental method

X-RAY DIFFRACTION (1.43 Å)