6FI7

E.coli Sigma factor S (RpoS) Region 4

Summary for 6FI7

• Entry DOI: 10.2210/pdb6fi7/pdb
• NMR Information: BMRB: 34234
• Descriptor: RNA polymerase sigma factor RpoS (1 entity in total)
• Functional Keywords: apo-rpos region 4, free rpos region4, rna binding protein
• Biological source: Escherichia coli
• Total number of polymer chains: 1
• Total formula weight: 10041.38

Authors

Liu, B.,Matthews, S.J. (deposition date: 2018-01-17, release date: 2018-06-06, Last modification date: 2024-06-19)

Primary citation

Tabib-Salazar, A.,Liu, B.,Barker, D.,Burchell, L.,Qimron, U.,Matthews, S.J.,Wigneshweraraj, S.
T7 phage factor required for managing RpoS inEscherichia coli.
Proc. Natl. Acad. Sci. U.S.A., 115:E5353-E5362, 2018

PubMed Abstract: T7 development in requires the inhibition of the housekeeping form of the bacterial RNA polymerase (RNAP), Eσ, by two T7 proteins: Gp2 and Gp5.7. Although the biological role of Gp2 is well understood, that of Gp5.7 remains to be fully deciphered. Here, we present results from functional and structural analyses to reveal that Gp5.7 primarily serves to inhibit Eσ, the predominant form of the RNAP in the stationary phase of growth, which accumulates in exponentially growing as a consequence of the buildup of guanosine pentaphosphate [(p)ppGpp] during T7 development. We further demonstrate a requirement of Gp5.7 for T7 development in cells in the stationary phase of growth. Our finding represents a paradigm for how some lytic phages have evolved distinct mechanisms to inhibit the bacterial transcription machinery to facilitate phage development in bacteria in the exponential and stationary phases of growth.

PubMed: 29789383
DOI: 10.1073/pnas.1800429115

Experimental method

SOLUTION NMR