6FG8
Crystal structure of the BIR3 - SERK1 complex from Arabidopsis thaliana.
Summary for 6FG8
| Entry DOI | 10.2210/pdb6fg8/pdb |
| Related | 4lsc 6FG7 |
| Descriptor | Somatic embryogenesis receptor kinase 1, Probable inactive receptor kinase At1g27190, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total) |
| Functional Keywords | leucine rich repeat receptor, membrane receptor, pseudokinase, ectodomain, receptor complex, negative regulator, protein binding |
| Biological source | Arabidopsis thaliana (Mouse-ear cress) More |
| Total number of polymer chains | 2 |
| Total formula weight | 58334.24 |
| Authors | Hothorn, M.,Hohmann, U. (deposition date: 2018-01-10, release date: 2018-01-24, Last modification date: 2024-01-17) |
| Primary citation | Hohmann, U.,Nicolet, J.,Moretti, A.,Hothorn, L.A.,Hothorn, M. The SERK3 elongated allele defines a role for BIR ectodomains in brassinosteroid signalling. Nat Plants, 4:345-351, 2018 Cited by PubMed Abstract: The leucine-rich repeat receptor kinase (LRR-RK) BRASSINOSTEROID INSENSITIVE 1 (BRI1) requires a shape-complementary SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) co-receptor for brassinosteroid sensing and receptor activation. Interface mutations that weaken the interaction between receptor and co-receptor in vitro reduce brassinosteroid signalling responses. The SERK3 elongated (elg) allele maps to the complex interface and shows enhanced brassinosteroid signalling, but surprisingly no tighter binding to the BRI1 ectodomain in vitro. Here, we report that rather than promoting the interaction with BRI1, the elg mutation disrupts the ability of the co-receptor to interact with the ectodomains of BRI1-ASSOCIATED-KINASE1 INTERACTING KINASE (BIR) receptor pseudokinases, negative regulators of LRR-RK signalling. A conserved lateral surface patch in BIR LRR domains is required for targeting SERK co-receptors and the elg allele maps to the core of the complex interface in a 1.25 Å BIR3-SERK1 structure. Collectively, our structural, quantitative biochemical and genetic analyses suggest that brassinosteroid signalling complex formation is negatively regulated by BIR receptor ectodomains. PubMed: 29735985DOI: 10.1038/s41477-018-0150-9 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.25 Å) |
Structure validation
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