6FFZ

Crystal structure of R. ruber ADH-A, mutant F43H, Y54L

6FFZ の概要

• エントリーDOI: 10.2210/pdb6ffz/pdb
• 分子名称: Alcohol dehydrogenase, ZINC ION, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ... (4 entities in total)
• 機能のキーワード: alcohol dehydrogenase mutant variant, nadh-dependent, zn2+-dependent, rossmann fold, oxidoreductase
• 由来する生物種: Rhodococcus sp. M8
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 147340.88

構造登録者

Dobritzsch, D.,Maurer, D.,Hamnevik, E.,Enugala, T.R.,Widersten, M. (登録日: 2018-01-09, 公開日: 2018-02-14, 最終更新日: 2024-01-17)

主引用文献

Hamnevik, E.,Maurer, D.,Enugala, T.R.,Chu, T.,Lofgren, R.,Dobritzsch, D.,Widersten, M.
Directed Evolution of Alcohol Dehydrogenase for Improved Stereoselective Redox Transformations of 1-Phenylethane-1,2-diol and Its Corresponding Acyloin.
Biochemistry, 57:1059-1062, 2018

PubMed Abstract: Laboratory evolution of alcohol dehydrogenase produced enzyme variants with improved turnover numbers with a vicinal 1,2-diol and its corresponding hydroxyketone. Crystal structure and transient kinetics analysis aids in rationalizing the new functions of these variants.

PubMed: 29384657
DOI: 10.1021/acs.biochem.8b00055

実験手法

X-RAY DIFFRACTION (1.71 Å)