6FFX

Crystal structure of R. ruber ADH-A, mutant F43H

Summary for 6FFX

• Entry DOI: 10.2210/pdb6ffx/pdb
• Descriptor: Alcohol dehydrogenase, ZINC ION, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ... (4 entities in total)
• Functional Keywords: alcohol dehydrogenase mutant variant, nadh-dependent, zn2+-dependent, rossmann fold, oxidoreductase
• Biological source: Rhodococcus sp. M8
• Total number of polymer chains: 4
• Total formula weight: 147540.94

Authors

Dobritzsch, D.,Maurer, D.,Hamnevik, E.,Enugala, T.R.,Widersten, M. (deposition date: 2018-01-09, release date: 2018-02-14, Last modification date: 2024-01-17)

Primary citation

Hamnevik, E.,Maurer, D.,Enugala, T.R.,Chu, T.,Lofgren, R.,Dobritzsch, D.,Widersten, M.
Directed Evolution of Alcohol Dehydrogenase for Improved Stereoselective Redox Transformations of 1-Phenylethane-1,2-diol and Its Corresponding Acyloin.
Biochemistry, 57:1059-1062, 2018

PubMed Abstract: Laboratory evolution of alcohol dehydrogenase produced enzyme variants with improved turnover numbers with a vicinal 1,2-diol and its corresponding hydroxyketone. Crystal structure and transient kinetics analysis aids in rationalizing the new functions of these variants.

PubMed: 29384657
DOI: 10.1021/acs.biochem.8b00055

Experimental method

X-RAY DIFFRACTION (2.5 Å)