6FF7

human Bact spliceosome core structure

6FF7 の概要

• エントリーDOI: 10.2210/pdb6ff7/pdb
• 関連するPDBエントリー: 6FF4
• EMDBエントリー: 4240
• 分子名称: RNA-binding motif protein, X-linked 2, SNW domain-containing protein 1, Pleiotropic regulator 1, ... (55 entities in total)
• 機能のキーワード: spliceosome, human, hela, bact, dynamics, splicing
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 61
• 化学式量合計: 3741132.24

構造登録者

Haselbach, D.,Komarov, I.,Agafonov, D.,Hartmuth, K.,Graf, B.,Kastner, B.,Luehrmann, R.,Stark, H. (登録日: 2018-01-03, 公開日: 2019-03-13, 最終更新日: 2024-05-08)

主引用文献

Haselbach, D.,Komarov, I.,Agafonov, D.E.,Hartmuth, K.,Graf, B.,Dybkov, O.,Urlaub, H.,Kastner, B.,Luhrmann, R.,Stark, H.
Structure and Conformational Dynamics of the Human Spliceosomal BactComplex.
Cell, 172:454-464.e11, 2018

PubMed Abstract: The spliceosome is a highly dynamic macromolecular complex that precisely excises introns from pre-mRNA. Here we report the cryo-EM 3D structure of the human B spliceosome at 3.4 Å resolution. In the B state, the spliceosome is activated but not catalytically primed, so that it is functionally blocked prior to the first catalytic step of splicing. The spliceosomal core is similar to the yeast B spliceosome; important differences include the presence of the RNA helicase aquarius and peptidyl prolyl isomerases. To examine the overall dynamic behavior of the purified spliceosome, we developed a principal component analysis-based approach. Calculating the energy landscape revealed eight major conformational states, which we refined to higher resolution. Conformational differences of the highly flexible structural components between these eight states reveal how spliceosomal components contribute to the assembly of the spliceosome, allowing it to generate a dynamic interaction network required for its subsequent catalytic activation.

PubMed: 29361316
DOI: 10.1016/j.cell.2018.01.010

実験手法

ELECTRON MICROSCOPY (4.5 Å)