6FDB

Positively supercharged variant of the computationally designed cage protein O3-33

6FDB の概要

• エントリーDOI: 10.2210/pdb6fdb/pdb
• 分子名称: Propanediol utilization protein (1 entity in total)
• 機能のキーワード: computationally designed cage protein, o3-33, de novo protein
• 由来する生物種: Salmonella choleraesuis
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 161066.98

構造登録者

Edwardson, T.,Mori, T.,Hilvert, D. (登録日: 2017-12-22, 公開日: 2018-08-22, 最終更新日: 2024-01-17)

主引用文献

Edwardson, T.G.W.,Mori, T.,Hilvert, D.
Rational Engineering of a Designed Protein Cage for siRNA Delivery.
J. Am. Chem. Soc., 140:10439-10442, 2018

PubMed Abstract: Oligonucleotide therapeutics have transformative potential in modern medicine but are poor drug candidates in themselves unless fitted with compensatory carrier systems. We describe a simple approach to transform a designed porous protein cage into a nucleic acid delivery vehicle. By introducing arginine mutations to the lumenal surface, a positively supercharged capsule is created, which can encapsidate oligonucleotides in vitro with high binding affinity. We demonstrate that the siRNA-loaded cage is taken up by mammalian cells and releases its cargo to induce RNAi and knockdown gene expression. These general concepts could also be applied to alternative scaffold designs, expediting the development of artificial protein cages toward delivery applications.

PubMed: 30091604
DOI: 10.1021/jacs.8b06442

実験手法

X-RAY DIFFRACTION (3.619 Å)