6FAR

Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-mannoimidazole

6FAR の概要

• エントリーDOI: 10.2210/pdb6far/pdb
• 関連するPDBエントリー: 6FAM
• 分子名称: Glycosyl hydrolase family 71, alpha-D-mannopyranose, (5R,6R,7S,8R)-5-(HYDROXYMETHYL)-5,6,7,8-TETRAHYDROIMIDAZO[1,2-A]PYRIDINE-6,7,8-TRIOL, ... (4 entities in total)
• 機能のキーワード: complex, gh99, hydrolase
• 由来する生物種: Bacteroides xylanisolvens XB1A
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44313.94

構造登録者

Fernandes, P.Z.,Petricevic, M.,Sobala, L.F.,Davies, G.J.,Williams, S.J. (登録日: 2017-12-16, 公開日: 2018-03-21, 最終更新日: 2024-01-17)

主引用文献

Fernandes, P.Z.,Petricevic, M.,Sobala, L.,Davies, G.J.,Williams, S.J.
Exploration of Strategies for Mechanism-Based Inhibitor Design for Family GH99 endo-alpha-1,2-Mannanases.
Chemistry, 24:7464-7473, 2018

PubMed Abstract: endo-α-1,2-Mannosidases and -mannanases, members of glycoside hydrolase family 99 (GH99), cleave α-Glc/Man-1,3-α-Man-OR structures within mammalian N-linked glycans and fungal α-mannan, respectively. They are proposed to act through a two-step mechanism involving a 1,2-anhydrosugar "epoxide" intermediate incorporating two conserved catalytic carboxylates. In the first step, one carboxylate acts as a general base to deprotonate the 2-hydroxy group adjacent to the fissile glycosidic bond, and the other provides general acid assistance to the departure of the aglycon. We report herein the synthesis of two inhibitors designed to interact with either the general base (α-mannosyl-1,3-(2-aminodeoxymannojirimycin), Man2NH DMJ) or the general acid (α-mannosyl-1,3-mannoimidazole, ManManIm). Modest affinities were observed for an endo-α-1,2-mannanase from Bacteroides thetaiotaomicron. Structural studies revealed that Man2NH DMJ binds like other iminosugar inhibitors, which suggests that the poor inhibition shown by this compound is not a result of a failure to achieve the expected interaction with the general base, but rather the reduction in basicity of the endocyclic nitrogen caused by introduction of a vicinal, protonated amine at C2. ManManIm binds with the imidazole headgroup distorted downwards, a result of an unfavourable interaction with a conserved active site tyrosine. This study has identified important limitations associated with mechanism-inspired inhibitor design for GH99 enzymes.

PubMed: 29508463
DOI: 10.1002/chem.201800435

実験手法

X-RAY DIFFRACTION (1.3 Å)