6FAI

Structure of a eukaryotic cytoplasmic pre-40S ribosomal subunit

6FAI の概要

• エントリーDOI: 10.2210/pdb6fai/pdb
• EMDBエントリー: 4214 4215 4216 4217 4218
• 分子名称: 40S ribosomal protein S27-A, Serine/threonine-protein kinase RIO2, 20S ribosomal RNA, ... (38 entities in total)
• 機能のキーワード: pre-40s ribosme, cryo-em, ribosome assembly, ribosomes, ribosome biogenesis, ribosome
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• 細胞内の位置: Cytoplasm : P35997 P40160 P32905 P33442 P25443 P05750 P0CX35 P26783 P0CX37 P26786 Q3E7X9 P0CX39 O13516 P0CX47 P48589 P05756 P06367 Q01855 P0CX51 P02407 P0CX55 P41057 P07280 P38701 P0C0V8 P0C0W1 P0CX29 P0CX31 Q3E792 P0CX33 P38011 Q99216 P38333 Q07381; Nucleus: P34078
• タンパク質・核酸の鎖数: 36
• 化学式量合計: 1411963.71

構造登録者

Scaiola, A.,Pena, C.,Weisser, M.,Boehringer, D.,Leibundgut, M.,Klingauf-Nerurkar, P.,Gerhardy, S.,Panse, V.G.,Ban, N. (登録日: 2017-12-15, 公開日: 2018-02-28, 最終更新日: 2024-05-15)

主引用文献

Scaiola, A.,Pena, C.,Weisser, M.,Bohringer, D.,Leibundgut, M.,Klingauf-Nerurkar, P.,Gerhardy, S.,Panse, V.G.,Ban, N.
Structure of a eukaryotic cytoplasmic pre-40S ribosomal subunit.
EMBO J., 37:-, 2018

PubMed Abstract: Final maturation of eukaryotic ribosomes occurs in the cytoplasm and requires the sequential removal of associated assembly factors and processing of the immature 20S pre-RNA Using cryo-electron microscopy (cryo-EM), we have determined the structure of a yeast cytoplasmic pre-40S particle in complex with Enp1, Ltv1, Rio2, Tsr1, and Pno1 assembly factors poised to initiate final maturation. The structure reveals that the pre-rRNA adopts a highly distorted conformation of its 3' major and 3' minor domains stabilized by the binding of the assembly factors. This observation is consistent with a mechanism that involves concerted release of the assembly factors orchestrated by the folding of the rRNA in the head of the pre-40S subunit during the final stages of maturation. Our results provide a structural framework for the coordination of the final maturation events that drive a pre-40S particle toward the mature form capable of engaging in translation.

PubMed: 29459436
DOI: 10.15252/embj.201798499

実験手法

ELECTRON MICROSCOPY (3.4 Å)