6F9C

Model of the Rift Valley fever virus glycoprotein hexamer type 1

6F9C の概要

• エントリーDOI: 10.2210/pdb6f9c/pdb
• EMDBエントリー: 4198
• 分子名称: Glycoprotein (2 entities in total)
• 機能のキーワード: enveloped virus, rvfv, glycoprotein, fusion protein, virus
• 由来する生物種: Rift valley fever virus (RVFV); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 490946.83

構造登録者

Halldorsson, S.,Bowden, T.A.,Huiskonen, J.T. (登録日: 2017-12-14, 公開日: 2018-01-31, 最終更新日: 2024-05-15)

主引用文献

Halldorsson, S.,Li, S.,Li, M.,Harlos, K.,Bowden, T.A.,Huiskonen, J.T.
Shielding and activation of a viral membrane fusion protein.
Nat Commun, 9:349-349, 2018

PubMed Abstract: Entry of enveloped viruses relies on insertion of hydrophobic residues of the viral fusion protein into the host cell membrane. However, the intermediate conformations during fusion remain unknown. Here, we address the fusion mechanism of Rift Valley fever virus. We determine the crystal structure of the Gn glycoprotein and fit it with the Gc fusion protein into cryo-electron microscopy reconstructions of the virion. Our analysis reveals how the Gn shields the hydrophobic fusion loops of the Gc, preventing premature fusion. Electron cryotomography of virions interacting with membranes under acidic conditions reveals how the fusogenic Gc is activated upon removal of the Gn shield. Repositioning of the Gn allows extension of Gc and insertion of fusion loops in the outer leaflet of the target membrane. These data show early structural transitions that enveloped viruses undergo during host cell entry and indicate that analogous shielding mechanisms are utilized across diverse virus families.

PubMed: 29367607
DOI: 10.1038/s41467-017-02789-2

実験手法

ELECTRON MICROSCOPY (8 Å)