6F93

Helicobacter pylori serine hydroxymethyl transferase in apo form

6F93 の概要

• エントリーDOI: 10.2210/pdb6f93/pdb
• 分子名称: Serine hydroxymethyltransferase (2 entities in total)
• 機能のキーワード: shmt, methyltransferase, h. pylori folate-sensitive fragile sites, folate cycle, transferase
• 由来する生物種: Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 93686.76

構造登録者

Sodolescu, A.,Dian, C.,Terradot, L.,Bouzhir-Sima, L.,Lestini, R.,Myllykallio, H.,Skouloubris, S.,Liebl, U. (登録日: 2017-12-13, 公開日: 2018-12-26, 最終更新日: 2024-01-17)

主引用文献

Sodolescu, A.,Dian, C.,Terradot, L.,Bouzhir-Sima, L.,Lestini, R.,Myllykallio, H.,Skouloubris, S.,Liebl, U.
Structural and functional insight into serine hydroxymethyltransferase from Helicobacter pylori.
PLoS ONE, 13:e0208850-e0208850, 2018

PubMed Abstract: Serine hydroxymethyltransferase (SHMT), encoded by the glyA gene, is a ubiquitous pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyzes the formation of glycine from serine. The thereby generated 5,10-methylene tetrahydrofolate (MTHF) is a major source of cellular one-carbon units and a key intermediate in thymidylate biosynthesis. While in virtually all eukaryotic and many bacterial systems thymidylate synthase ThyA, SHMT and dihydrofolate reductase (DHFR) are part of the thymidylate/folate cycle, the situation is different in organisms using flavin-dependent thymidylate synthase ThyX. Here the distinct catalytic reaction directly produces tetrahydrofolate (THF) and consequently in most ThyX-containing organisms, DHFR is absent. While the resulting influence on the folate metabolism of ThyX-containing bacteria is not fully understood, the presence of ThyX may provide growth benefits under conditions where the level of reduced folate derivatives is compromised. Interestingly, the third key enzyme implicated in generation of MTHF, serine hydroxymethyltransferase (SHMT), has a universal phylogenetic distribution, but remains understudied in ThyX-containg bacteria. To obtain functional insight into these ThyX-dependent thymidylate/folate cycles, we characterized the predicted SHMT from the ThyX-containing bacterium Helicobacter pylori. Serine hydroxymethyltransferase activity was confirmed by functional genetic complementation of a glyA-inactivated E. coli strain. A H. pylori ΔglyA strain was obtained, but exhibited markedly slowed growth and had lost the virulence factor CagA. Biochemical and spectroscopic evidence indicated formation of a characteristic enzyme-PLP-glycine-folate complex and revealed unexpectedly weak binding affinity of PLP. The three-dimensional structure of the H. pylori SHMT apoprotein was determined at 2.8Ǻ resolution, suggesting a structural basis for the low affinity of the enzyme for its cofactor. Stabilization of the proposed inactive configuration using small molecules has potential to provide a specific way for inhibiting HpSHMT.

PubMed: 30550583
DOI: 10.1371/journal.pone.0208850

実験手法

X-RAY DIFFRACTION (2.8 Å)