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6F8S

Toxin-Antitoxin complex GraTA

6F8S の概要
エントリーDOI10.2210/pdb6f8s/pdb
分子名称XRE family transcriptional regulator, Putative Killer protein, SULFATE ION, ... (5 entities in total)
機能のキーワードgraa, grat, higa, higb, grata, higba, toxin
由来する生物種Pseudomonas putida (Arthrobacter siderocapsulatus)
詳細
タンパク質・核酸の鎖数4
化学式量合計44928.75
構造登録者
Talavera, A.,Loris, R. (登録日: 2017-12-13, 公開日: 2018-07-11, 最終更新日: 2024-05-08)
主引用文献Talavera, A.,Tamman, H.,Ainelo, A.,Konijnenberg, A.,Hadzi, S.,Sobott, F.,Garcia-Pino, A.,Horak, R.,Loris, R.
A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.
Nat Commun, 10:972-972, 2019
Cited by
PubMed Abstract: Bacterial toxin-antitoxin (TA) modules are tightly regulated to maintain growth in favorable conditions or growth arrest during stress. A typical regulatory strategy involves the antitoxin binding and repressing its own promoter while the toxin often acts as a co-repressor. Here we show that Pseudomonas putida graTA-encoded antitoxin GraA and toxin GraT differ from other TA proteins in the sense that not the antitoxin but the toxin possesses a flexible region. GraA auto-represses the graTA promoter: two GraA dimers bind cooperatively at opposite sides of the operator sequence. Contrary to other TA modules, GraT is a de-repressor of the graTA promoter as its N-terminal disordered segment prevents the binding of the GraTA complex to the operator. Removal of this region restores operator binding and abrogates Gr aT toxicity. GraTA represents a TA module where a flexible region in the toxin rather than in the antitoxin controls operon expression and toxin activity.
PubMed: 30814507
DOI: 10.1038/s41467-019-08865-z
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (2.5 Å)
構造検証レポート
Validation report summary of 6f8s
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-23に公開中

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