6F8S
Toxin-Antitoxin complex GraTA
6F8S の概要
エントリーDOI | 10.2210/pdb6f8s/pdb |
分子名称 | XRE family transcriptional regulator, Putative Killer protein, SULFATE ION, ... (5 entities in total) |
機能のキーワード | graa, grat, higa, higb, grata, higba, toxin |
由来する生物種 | Pseudomonas putida (Arthrobacter siderocapsulatus) 詳細 |
タンパク質・核酸の鎖数 | 4 |
化学式量合計 | 44928.75 |
構造登録者 | |
主引用文献 | Talavera, A.,Tamman, H.,Ainelo, A.,Konijnenberg, A.,Hadzi, S.,Sobott, F.,Garcia-Pino, A.,Horak, R.,Loris, R. A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nat Commun, 10:972-972, 2019 Cited by PubMed Abstract: Bacterial toxin-antitoxin (TA) modules are tightly regulated to maintain growth in favorable conditions or growth arrest during stress. A typical regulatory strategy involves the antitoxin binding and repressing its own promoter while the toxin often acts as a co-repressor. Here we show that Pseudomonas putida graTA-encoded antitoxin GraA and toxin GraT differ from other TA proteins in the sense that not the antitoxin but the toxin possesses a flexible region. GraA auto-represses the graTA promoter: two GraA dimers bind cooperatively at opposite sides of the operator sequence. Contrary to other TA modules, GraT is a de-repressor of the graTA promoter as its N-terminal disordered segment prevents the binding of the GraTA complex to the operator. Removal of this region restores operator binding and abrogates Gr aT toxicity. GraTA represents a TA module where a flexible region in the toxin rather than in the antitoxin controls operon expression and toxin activity. PubMed: 30814507DOI: 10.1038/s41467-019-08865-z 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (2.5 Å) |
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