6F8E

PH domain from TgAPH

Summary for 6F8E

• Entry DOI: 10.2210/pdb6f8e/pdb
• NMR Information: BMRB: 34216
• Descriptor: Pleckstrin homology domain (1 entity in total)
• Functional Keywords: ph domain, phosphatidic acid binding, membrane association, lipid binding protein
• Biological source: Toxoplasma gondii (strain ATCC 50611 / Me49)
• Total number of polymer chains: 1
• Total formula weight: 13759.89

Authors

Darvill, N.,Liu, B.,Matthews, S.,Soldati-Favre, D.,Rouse, S.,Benjamin, S.,Blake, T.,Dubois, D.J.,Hammoudi, P.M.,Pino, P. (deposition date: 2017-12-13, release date: 2019-01-30, Last modification date: 2024-06-19)

Primary citation

Darvill, N.,Dubois, D.J.,Rouse, S.L.,Hammoudi, P.M.,Blake, T.,Benjamin, S.,Liu, B.,Soldati-Favre, D.,Matthews, S.
Structural Basis of Phosphatidic Acid Sensing by APH in Apicomplexan Parasites.
Structure, 26:1059-1071.e6, 2018

PubMed Abstract: Plasmodium falciparum and Toxoplasma gondii are obligate intracellular parasites that belong to the phylum of Apicomplexa and cause major human diseases. Their access to an intracellular lifestyle is reliant on the coordinated release of proteins from the specialized apical organelles called micronemes and rhoptries. A specific phosphatidic acid effector, the acylated pleckstrin homology domain-containing protein (APH) plays a central role in microneme exocytosis and thus is essential for motility, cell entry, and egress. TgAPH is acylated on the surface of the micronemes and recruited to phosphatidic acid (PA)-enriched membranes. Here, we dissect the atomic details of APH PA-sensing hub and its functional interaction with phospholipid membranes. We unravel the key determinant of PA recognition for the first time and show that APH inserts into and clusters multiple phosphate head-groups at the bilayer binding surface.

PubMed: 29910186
DOI: 10.1016/j.str.2018.05.001

Experimental method

SOLUTION NMR