6F5Z

Complex between the Haloferax volcanii Trm112 methyltransferase activator and the Hvo_0019 putative methyltransferase

6F5Z の概要

• エントリーDOI: 10.2210/pdb6f5z/pdb
• 分子名称: 24-sterol C-methyltransferase, UPF0434 family protein, S-ADENOSYL-L-HOMOCYSTEINE, ... (5 entities in total)
• 機能のキーワード: protein complex, holoenzyme, methyltransferase, halophile, archaea, transferase
• 由来する生物種: Haloferax volcanii (Halobacterium volcanii); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 67283.75

構造登録者

Graille, M.,van Tran, N. (登録日: 2017-12-04, 公開日: 2018-07-11, 最終更新日: 2025-12-10)

主引用文献

van Tran, N.,Muller, L.,Ross, R.L.,Lestini, R.,Letoquart, J.,Ulryck, N.,Limbach, P.A.,de Crecy-Lagard, V.,Cianferani, S.,Graille, M.
Evolutionary insights into Trm112-methyltransferase holoenzymes involved in translation between archaea and eukaryotes.
Nucleic Acids Res., 46:8483-8499, 2018

PubMed Abstract: Protein synthesis is a complex and highly coordinated process requiring many different protein factors as well as various types of nucleic acids. All translation machinery components require multiple maturation events to be functional. These include post-transcriptional and post-translational modification steps and methylations are the most frequent among these events. In eukaryotes, Trm112, a small protein (COG2835) conserved in all three domains of life, interacts and activates four methyltransferases (Bud23, Trm9, Trm11 and Mtq2) that target different components of the translation machinery (rRNA, tRNAs, release factors). To clarify the function of Trm112 in archaea, we have characterized functionally and structurally its interaction network using Haloferax volcanii as model system. This led us to unravel that methyltransferases are also privileged Trm112 partners in archaea and that this Trm112 network is much more complex than anticipated from eukaryotic studies. Interestingly, among the identified enzymes, some are functionally orthologous to eukaryotic Trm112 partners, emphasizing again the similarity between eukaryotic and archaeal translation machineries. Other partners display some similarities with bacterial methyltransferases, suggesting that Trm112 is a general partner for methyltransferases in all living organisms.

PubMed: 30010922
DOI: 10.1093/nar/gky638

実験手法

X-RAY DIFFRACTION (1.35 Å)