6F4H

Crystal structure of the Drosophila melanogaster SNF/U1-SL2 complex

6F4H の概要

• エントリーDOI: 10.2210/pdb6f4h/pdb
• 分子名称: U1 small nuclear ribonucleoprotein A, U1-SL2, CITRATE ANION, ... (4 entities in total)
• 機能のキーワード: snf, u2a' rrm, splicing, evolution
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 54435.74

構造登録者

Weber, G.,Holton, N.,Hall, K.B.,DeKoster, G.,Wahl, M.C. (登録日: 2017-11-29, 公開日: 2018-06-20, 最終更新日: 2024-05-08)

主引用文献

Weber, G.,DeKoster, G.T.,Holton, N.,Hall, K.B.,Wahl, M.C.
Molecular principles underlying dual RNA specificity in the Drosophila SNF protein.
Nat Commun, 9:2220-2220, 2018

PubMed Abstract: The first RNA recognition motif of the Drosophila SNF protein is an example of an RNA binding protein with multi-specificity. It binds different RNA hairpin loops in spliceosomal U1 or U2 small nuclear RNAs, and only in the latter case requires the auxiliary U2A' protein. Here we investigate its functions by crystal structures of SNF alone and bound to U1 stem-loop II, U2A' or U2 stem-loop IV and U2A', SNF dynamics from NMR spectroscopy, and structure-guided mutagenesis in binding studies. We find that different loop-closing base pairs and a nucleotide exchange at the tips of the loops contribute to differential SNF affinity for the RNAs. U2A' immobilizes SNF and RNA residues to restore U2 stem-loop IV binding affinity, while U1 stem-loop II binding does not require such adjustments. Our findings show how U2A' can modulate RNA specificity of SNF without changing SNF conformation or relying on direct RNA contacts.

PubMed: 29880797
DOI: 10.1038/s41467-018-04561-6

実験手法

X-RAY DIFFRACTION (2 Å)