6F07

CBF3 Core Complex

6F07 の概要

• エントリーDOI: 10.2210/pdb6f07/pdb
• EMDBエントリー: 4163
• 分子名称: Centromere DNA-binding protein complex CBF3 subunit B, Suppressor of kinetochore protein 1, ZINC ION (3 entities in total)
• 機能のキーワード: kinetochore, f-box, dna-binding, centromere, cell cycle
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 167762.32

構造登録者

Leber, V.,Singleton, M.R. (登録日: 2017-11-17, 公開日: 2017-12-13, 最終更新日: 2024-10-09)

主引用文献

Leber, V.,Nans, A.,Singleton, M.R.
Structural basis for assembly of the CBF3 kinetochore complex.
EMBO J., 37:269-281, 2018

PubMed Abstract: Eukaryotic chromosomes contain a specialised region known as the centromere, which forms the platform for kinetochore assembly and microtubule attachment. The centromere is distinguished by the presence of nucleosomes containing the histone H3 variant, CENP-A. In budding yeast, centromere establishment begins with the recognition of a specific DNA sequence by the CBF3 complex. This in turn facilitates CENP-A nucleosome deposition and kinetochore assembly. Here, we describe a 3.6 Å single-particle cryo-EM reconstruction of the core CBF3 complex, incorporating the sequence-specific DNA-binding protein Cep3 together with regulatory subunits Ctf13 and Skp1. This provides the first structural data on Ctf13, defining it as an F-box protein of the leucine-rich-repeat family, and demonstrates how a novel F-box-mediated interaction between Ctf13 and Skp1 is responsible for initial assembly of the CBF3 complex.

PubMed: 29212814
DOI: 10.15252/embj.201798134

実験手法

ELECTRON MICROSCOPY (3.6 Å)