6EZV

The cytotoxin MakA from Vibrio cholerae

Summary for 6EZV

• Entry DOI: 10.2210/pdb6ezv/pdb
• Descriptor: Non-hemolytic enterotoxin lytic component L1, GLYCEROL, ACETATE ION, ... (5 entities in total)
• Functional Keywords: cytotoxin, vibrio cholerae, type-3 secretion, flagella, toxin
• Biological source: Vibrio cholerae
• Total number of polymer chains: 1
• Total formula weight: 42658.35

Authors

Persson, K.,Dongre, M.,Wai, S.N. (deposition date: 2017-11-16, release date: 2018-05-02, Last modification date: 2024-05-08)

Primary citation

Dongre, M.,Singh, B.,Aung, K.M.,Larsson, P.,Miftakhova, R.,Persson, K.,Askarian, F.,Johannessen, M.,von Hofsten, J.,Persson, J.L.,Erhardt, M.,Tuck, S.,Uhlin, B.E.,Wai, S.N.
Flagella-mediated secretion of a novelVibrio choleraecytotoxin affecting both vertebrate and invertebrate hosts.
Commun Biol, 1:59-59, 2018

PubMed Abstract: Using as an infection host model for predator interactions, we discovered a bacterial cytotoxin, MakA, whose function as a virulence factor relies on secretion via the flagellum channel in a proton motive force-dependent manner. The MakA protein is expressed from the polycistronic (otility-ssociated lling factor) operon. Bacteria expressing induced dramatic changes in intestinal morphology leading to a defecation defect, starvation and death in . The Mak proteins also promoted colonization of the zebrafish gut causing lethal infection. A structural model of purified MakA at 1.9 Å resolution indicated similarities to members of a superfamily of bacterial toxins with unknown biological roles. Our findings reveal an unrecognized role for flagella in cytotoxin export that may contribute both to environmental spread of the bacteria by promoting survival and proliferation in encounters with predators, and to pathophysiological effects during infections.

PubMed: 30271941
DOI: 10.1038/s42003-018-0065-z

Experimental method

X-RAY DIFFRACTION (1.9 Å)