6EZV

The cytotoxin MakA from Vibrio cholerae

6EZV の概要

• エントリーDOI: 10.2210/pdb6ezv/pdb
• 分子名称: Non-hemolytic enterotoxin lytic component L1, GLYCEROL, ACETATE ION, ... (5 entities in total)
• 機能のキーワード: cytotoxin, vibrio cholerae, type-3 secretion, flagella, toxin
• 由来する生物種: Vibrio cholerae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42658.35

構造登録者

Persson, K.,Dongre, M.,Wai, S.N. (登録日: 2017-11-16, 公開日: 2018-05-02, 最終更新日: 2024-05-08)

主引用文献

Dongre, M.,Singh, B.,Aung, K.M.,Larsson, P.,Miftakhova, R.,Persson, K.,Askarian, F.,Johannessen, M.,von Hofsten, J.,Persson, J.L.,Erhardt, M.,Tuck, S.,Uhlin, B.E.,Wai, S.N.
Flagella-mediated secretion of a novelVibrio choleraecytotoxin affecting both vertebrate and invertebrate hosts.
Commun Biol, 1:59-59, 2018

PubMed Abstract: Using as an infection host model for predator interactions, we discovered a bacterial cytotoxin, MakA, whose function as a virulence factor relies on secretion via the flagellum channel in a proton motive force-dependent manner. The MakA protein is expressed from the polycistronic (otility-ssociated lling factor) operon. Bacteria expressing induced dramatic changes in intestinal morphology leading to a defecation defect, starvation and death in . The Mak proteins also promoted colonization of the zebrafish gut causing lethal infection. A structural model of purified MakA at 1.9 Å resolution indicated similarities to members of a superfamily of bacterial toxins with unknown biological roles. Our findings reveal an unrecognized role for flagella in cytotoxin export that may contribute both to environmental spread of the bacteria by promoting survival and proliferation in encounters with predators, and to pathophysiological effects during infections.

PubMed: 30271941
DOI: 10.1038/s42003-018-0065-z

実験手法

X-RAY DIFFRACTION (1.9 Å)