6ETR

Atomic resolution structure of RNase A (data collection 8)

6ETR の概要

• エントリーDOI: 10.2210/pdb6etr/pdb
• 分子名称: Ribonuclease pancreatic, ISOPROPYL ALCOHOL (3 entities in total)
• 機能のキーワード: raman microspectroscopy, ribonuclease, atomic resolution, radiation damage, photodamage, hydrolase
• 由来する生物種: Bos taurus (Bovine)
• 細胞内の位置: Secreted: P61823
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13888.61

構造登録者

Caterino, M.,Vergara, A.,Merlino, A. (登録日: 2017-10-27, 公開日: 2018-02-21, 最終更新日: 2024-11-06)

主引用文献

Vergara, A.,Caterino, M.,Merlino, A.
Raman-markers of X-ray radiation damage of proteins.
Int. J. Biol. Macromol., 111:1194-1205, 2018

PubMed Abstract: Despite their high relevance, the mechanisms of X-ray radiation damage on protein structure yet have to be completely established. Here, we used Raman microspectrophotometry to follow X-ray-induced chemical modifications on the structure of the model protein bovine pancreatic ribonuclease (RNase A). The combination of dose-dependent Raman spectra and ultrahigh resolution (eight structures solved using data collected between 0.85 and 1.17 Å resolution on the same single crystal) allowed direct observation of several radiation damage events, including covalent bond breakages and formation of radicals. Our results are relevant for analytical photodamage detection and provide implications for a detailed understanding of the mechanisms of photoproduct formation.

PubMed: 29374529
DOI: 10.1016/j.ijbiomac.2018.01.135

実験手法

X-RAY DIFFRACTION (1.17 Å)