6ERL

Quadruplex with flipped tetrad formed by the c-myc promoter sequence

6ERL の概要

• エントリーDOI: 10.2210/pdb6erl/pdb
• NMR情報: BMRB: 34186
• 分子名称: cmbr-481317 (1 entity in total)
• 機能のキーワード: quadruplex, g4, 8-bromo-guanosine, tetrad flip, dna
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7324.09

構造登録者

Karg, B.,Weisz, K. (登録日: 2017-10-18, 公開日: 2018-06-13, 最終更新日: 2024-06-19)

主引用文献

Karg, B.,Weisz, K.
Loop Length Affects Syn-Anti Conformational Rearrangements in Parallel G-Quadruplexes.
Chemistry, 2018

PubMed Abstract: A G-quadruplex forming sequence from the MYC promoter region was modified with syn-favoring 8-bromo-2'-deoxyguanosine residues. Depending on the number and position of modifications in the intramolecular parallel G-quadruplex, substitutions with the bromoguanosine analogue at the 5'-tetrad induce conformational rearrangements with concerted all-anti to all-syn transitions for all residues of the modified G-quartet. No unfavorable steric interactions of the C8-substituents in the medium grooves are apparent in the high-resolution structure as determined for a tetrasubstituted MYC quadruplex that exclusively forms the all-syn isomer. In contrast, considerable steric clashes with 5'-phosphate oxygen atoms for those analogues that follow a less flexible 1-nucleotide loop in the native all-anti conformation seem to constitute the major driving force for the tetrad inversion and allow for the rational design of appropriately substituted sequences. Correlations found between the population of species subjected to a tetrad flip and melting temperatures indicate that more effective conformational transitions are compromised by lower thermal stabilities of the modified parallel quadruplexes.

PubMed: 29756658
DOI: 10.1002/chem.201801851

実験手法

SOLUTION NMR