6ERE

Crystal structure of a computationally designed colicin endonuclease and immunity pair colEdes3/Imdes3

6ERE の概要

• エントリーDOI: 10.2210/pdb6ere/pdb
• 関連するPDBエントリー: 6ER6
• 分子名称: colicin, Immunity, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: immunity colicin, immune system
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 53072.37

構造登録者

Netzer, R.,Listov, D.,Dym, O.,Albeck, S.,Knop, O.,Fleishman, S.J. (登録日: 2017-10-18, 公開日: 2019-01-30, 最終更新日: 2024-01-17)

主引用文献

Netzer, R.,Listov, D.,Lipsh, R.,Dym, O.,Albeck, S.,Knop, O.,Kleanthous, C.,Fleishman, S.J.
Ultrahigh specificity in a network of computationally designed protein-interaction pairs.
Nat Commun, 9:5286-5286, 2018

PubMed Abstract: Protein networks in all organisms comprise homologous interacting pairs. In these networks, some proteins are specific, interacting with one or a few binding partners, whereas others are multispecific and bind a range of targets. We describe an algorithm that starts from an interacting pair and designs dozens of new pairs with diverse backbone conformations at the binding site as well as new binding orientations and sequences. Applied to a high-affinity bacterial pair, the algorithm results in 18 new ones, with cognate affinities from pico- to micromolar. Three pairs exhibit 3-5 orders of magnitude switch in specificity relative to the wild type, whereas others are multispecific, collectively forming a protein-interaction network. Crystallographic analysis confirms design accuracy, including in new backbones and polar interactions. Preorganized polar interaction networks are responsible for high specificity, thus defining design principles that can be applied to program synthetic cellular interaction networks of desired affinity and specificity.

PubMed: 30538236
DOI: 10.1038/s41467-018-07722-9

実験手法

X-RAY DIFFRACTION (2.25 Å)